About: Detection and analysis system for hybridization images of lab-in-a-tube microarray

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection and analysis system for hybridization images of lab-in-a-tube microarray
Creator	Liu, Quanjun Qin, Zhou Yunfei, Bai Zuhong, L
Source	PMC
abstract	A lab-in-a-tube microarray system is developed for sample inspection and signal detection by fabricating a flat transparent window cap of the Eppendorf tube. The oligonucleotide microarray is immobilized on the inner surface of the cap. A small vessel is placed in an Eppendorf tube for storing hybridization solutions. With the microarray system, the full biochemical processes, including gene fragment amplification, fluorescence labeling, hybridization, and fluorescence detection, have been performed in the sealed tube without opening the cap. The images are obtained from a fluorescence microscope and captured by a CCD, and the data are transported to a computer through the universal serial bus (USB). After noise reduction, signal intensity is determined from hybridization image and the presence of gene fragments is identified. The final data output includes sample information, process steps, and hybridization results. A lab-in-a-tube microarray system for detecting ten respiratory viruses at a single detection is designed. High detection throughput and accuracy have been demonstrated with the system.
has issue date	2005-01-01(xsd:dateTime)
bibo:doi	10.1360/982005-451
has license	no-cc
sha1sum (hex)	c414d47ea151fe1e91600dd24fa0e330b645d229
schema:url	https://doi.org/10.1360/982005-451
resource representing a document's title	Detection and analysis system for hybridization images of lab-in-a-tube microarray
has PubMed Central identifier	PMC7088752
schema:publication	Chin Sci Bull
resource representing a document's body	covid:c414d47ea151fe1e91600dd24fa0e330b645d229#body_text
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