About: Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation

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About: Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation
Creator	Chen, Huanchun Fang, Liurong Fang, Ying Lei, Yingying Luo, Rui Wang, Dang Wang, Yang Xiao, Shaobo
Source	Medline; PMC
abstract	BACKGROUND: Nuclear factor-kappaB (NF-κB) is an inducible transcription factor that plays a key role in inflammation and immune responses, as well as in the regulation of cell proliferation and survival. Previous studies by our group and others have demonstrated that porcine reproductive and respiratory syndrome virus (PRRSV) infection could activate NF-κB in MARC-145 cells and alveolar macrophages. The nucleocapsid (N) protein was identified as an NF-κB activator among the structural proteins encoded by PRRSV; however, it remains unclear whether the nonstructural proteins (Nsps) contribute to NF-κB activation. In this study, we identified which Nsps can activate NF-κB and investigated the potential mechanism(s) by which they act. RESULTS: By screening the individual Nsps of PRRSV strain WUH3, Nsp2 exhibited great potential to activate NF-κB in MARC-145 and HeLa cells. Overexpression of Nsp2 induced IκBα degradation and nuclear translocation of NF-κB. Furthermore, Nsp2 also induced NF-κB-dependent inflammatory factors, including interleukin (IL)-6, IL-8, COX-2, and RANTES. Compared with the Nsp2 of the classical PRRSV strain, the Nsp2 of highly pathogenic PRRSV (HP-PRRSV) strains that possess a 30 amino acid (aa) deletion in Nsp2 displayed greater NF-κB activation. However, the 30-aa deletion was demonstrated to not be associated with NF-κB activation. Further functional domain analyses revealed that the hypervariable region (HV) of Nsp2 was essential for NF-κB activation. CONCLUSIONS: Taken together, these data indicate that PRRSV Nsp2 is a multifunctional protein participating in the modulation of host inflammatory response, which suggests an important role of Nsp2 in pathogenesis and disease outcomes.
has issue date	2012-04-30(xsd:dateTime)
bibo:doi	10.1186/1743-422x-9-83
bibo:pmid	22546080
has license	cc-by
sha1sum (hex)	c0f496f49f4b2dea73cf8c184ff21275bf706908
schema:url	https://doi.org/10.1186/1743-422x-9-83
resource representing a document's title	Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation
has PubMed Central identifier	PMC3443020
has PubMed identifier	22546080
schema:publication	Virol J
resource representing a document's body	covid:c0f496f49f4b2dea73cf8c184ff21275bf706908#body_text
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