About: Murine monoclonal antibody which can distinguish cystatins SA1 and SA2

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About: Murine monoclonal antibody which can distinguish cystatins SA1 and SA2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Murine monoclonal antibody which can distinguish cystatins SA1 and SA2
Creator	Ito, Taichi Kato, Tetsuo Komiya-Ito, Akiyo Minaguchi, Kiyoshi Okuda, Katsuji Saitoh, Eiichi Yamada, Satoru
Source	Elsevier; Medline; PMC
abstract	Abstract To develop a diagnostic trial enabling the selective examination for a target cystatin in human body fluids, we attempted to prepare monoclonal antibodies against human cystatin SA1 (originally cystatin SA) and its variant form (cystatin SA2). BALB/c mice were immunized with recombinant (r-) cystatins SA1 and SA2. Two monoclonal antibodies designated Cys3F11 and Cys2E5 were selected. By ELISA analyses, the Cys2E5 was shown to react with r-cystatin SA2 but also somewhat with r-cystatin SA1 (22% cross-reactivity) and with plasma cystatin C (18% cross-reactivity), indicating a high specificity for cystatin SA2. The Cys3F11 reacted not only with r-cystatin SA1 but also with r-cystatin SA2 (89% cross-reactivity) and plasma cystatin C (47% cross-reactivity). This finding was further emphasized by immunoblotting of human submandibular–sublingual saliva samples. ELISA additivity test suggests that the two monoclonal antibodies bind to distinct epitopes. In conclusion, we have succeeded in producing two antibodies that discriminate the structural differences between salivary cystatins S and SN, which share more than 90% identity in amino acid sequence with cystatin SA.
has issue date	2005-06-30(xsd:dateTime)
bibo:doi	10.1016/j.molimm.2004.11.021
bibo:pmid	15829315
has license	els-covid
sha1sum (hex)	bf9bbb32570e5c653011e31c67cf5fb415ffa967
schema:url	https://doi.org/10.1016/j.molimm.2004.11.021
resource representing a document's title	Murine monoclonal antibody which can distinguish cystatins SA1 and SA2
has PubMed Central identifier	PMC7126565
has PubMed identifier	15829315
schema:publication	Molecular Immunology
resource representing a document's body	covid:bf9bbb32570e5c653011e31c67cf5fb415ffa967#body_text
is schema:about of	named entity 'sequence' named entity 'cystatin' named entity 'This' named entity 'human body' named entity 'mice' named entity 'emphasized' named entity 'test' named entity 'MONOCLONAL ANTIBODY' named entity 'BODY FLUIDS' named entity 'SUBLINGUAL' named entity 'SOMEWHAT' named entity 'EPITOPES' named entity 'MORE THAN' named entity 'BIND' named entity 'HUMAN BODY' named entity 'BUT' named entity 'IMMUNOBLOTTING' named entity 'HUMAN' named entity 'SELECTED' named entity '90%' named entity 'STRUCTURAL' named entity 'IMMUNIZED' named entity 'ANTIBODIES' named entity 'PLASMA' named entity 'SELECTIVE' named entity 'CYSTATIN SA' named entity 'BALB' named entity 'MICE' named entity 'CYSTATIN' named entity 'SA1' named entity 'TEST' named entity 'MURINE' named entity 'CYSTATINS' named entity 'DISTINCT' named entity 'ELISA' named entity 'FINDING' named entity 'CYSTATINS' named entity 'ENABLING' named entity 'SAMPLES' named entity 'EXAMINATION' named entity 'REACTIVITY' named entity 'PRODUCING' named entity 'SUBMANDIBULAR' named entity 'DISCRIMINATE' named entity 'CONCLUSION' named entity 'SA1' named entity 'SA2' named entity 'DESIGNATED' named entity 'AMINO ACID SEQUENCE' named entity 'TARGET' named entity '2818' named entity 'CROSS' named entity 'ITS' named entity 'REACT' named entity '2889' named entity 'IDENTITY' named entity 'TO PREPARE' named entity 'SPECIFICITY' named entity 'RECOMBINANT' named entity 'SALIVARY CYSTATINS' named entity 'VARIANT' named entity 'SHARE' named entity 'FORM' named entity 'MONOCLONAL ANTIBODIES' named entity 'INDICATING' named entity 'DIAGNOSTIC TRIAL' named entity 'ATTEMPTED' named entity 'HIGH' named entity 'SALIVA' named entity 'HAVE' named entity 'SA2' named entity 'CYSTATIN C' named entity 'SUCCEEDED' covid:arg/bf9bbb32570e5c653011e31c67cf5fb415ffa967 named entity 'enabling'

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