About: An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

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About: An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
Creator	Huang, Guangrui Xu, Anlong Chen, Shangwu Lan, Dongming Shao, Hongwei Zhang, Lichun Ma, Lixin
Source	Elsevier; Medline; PMC
abstract	Abstract Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefore, an additional chromatographic purification step is required to remove the proteases, and this is time- and labor-consuming. Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography.
has issue date	2011-08-15(xsd:dateTime)
bibo:doi	10.1016/j.ab.2011.04.034
bibo:pmid	21600185
has license	els-covid
sha1sum (hex)	bd4e499e67b4c48f39e88d7f0faee97eed30596a
schema:url	https://doi.org/10.1016/j.ab.2011.04.034
resource representing a document's title	An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
has PubMed Central identifier	PMC7094418
has PubMed identifier	21600185
schema:publication	Analytical Biochemistry
resource representing a document's body	covid:bd4e499e67b4c48f39e88d7f0faee97eed30596a#body_text
is schema:about of	named entity 'chromatography' named entity 'tag' named entity 'ELP' named entity 'TAGGED' named entity 'HERE' named entity 'PURIFICATION' named entity 'TO AVOID' named entity 'ELP' named entity 'ELASTIN' named entity 'CLEAVAGE' named entity 'SEPARATED FROM' named entity 'CHROMATOGRAPHY' named entity 'POLYPEPTIDE' named entity 'LIKE' named entity 'CLEAVED' named entity 'ALLOWING' named entity 'ADDITIONAL' named entity 'TAG' named entity 'POLYPEPTIDE' named entity 'PURIFICATION' named entity 'PROTEINS' named entity 'EXTRACT' named entity 'FUSED TO' named entity 'INTERFERENCE' named entity 'METHOD' named entity 'STEP' named entity 'TARGET PROTEIN' named entity 'REQUIRED' named entity 'DIGESTION' named entity 'USING' named entity 'LIKE' named entity 'RECOMBINANT PROTEINS' named entity 'IMPROVED' named entity 'PROTEASES' named entity 'UTILITY' named entity 'CELL' named entity 'NECESSARY' named entity 'TIME' named entity 'PROTEASE' named entity 'FUSION' named entity 'REMOVAL' named entity 'TO REMOVE' named entity 'PROTEIN ' named entity 'TAGGED' named entity 'ELASTIN' named entity 'PROTEASES' named entity 'PROPERTIES' covid:arg/bd4e499e67b4c48f39e88d7f0faee97eed30596a named entity 'target' named entity 'cell' named entity 'improved' named entity 'proteases' named entity 'ELP' named entity 'target protein' named entity 'digestion' named entity 'cleavage' named entity 'fusion proteins' named entity 'centrifugation' named entity 'protease' named entity 'GFP' named entity 'SDS-PAGE' named entity 'cleavage' named entity 'thioredoxin' named entity 'phase transition' named entity '17.1' named entity 'ELP' named entity 'elastin' named entity 'protease' named entity 'ELP' named entity 'protease' named entity 'GFP' named entity 'protease' named entity 'Escherichia coli' named entity 'SDS-PAGE' named entity 'recombinant proteins'

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