About: Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein

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About: Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein
Creator	Li, Shih-Wein Lin, Cheng-Wen Wan, Lei Lu, Kai-Zen Yang, Tsuey-Ching Hsieh, Tsung-Han Lai, Chien-Chen Chiu, Man-Tzu
Source	Medline; PMC
abstract	Japanese encephalitis virus (JEV) nonstructural protein 5 (NS5) exhibits a Type I interferon (IFN) antagonistic function. This study characterizes Type I IFN antagonism mechanism of NS5 protein, using proteomic approach. In human neuroblastoma cells, NS5 expression would suppress IFNβ‐induced responses, for example, expression of IFN‐stimulated genes PKR and OAS as well as STAT1 nuclear translocation and phosphorylation. Proteomic analysis showed JEV NS5 downregulating calreticulin, while upregulating cyclophilin A, HSP 60 and stress‐induced‐phosphoprotein 1. Gene silence of calreticulin raised intracellular Ca(2+) levels while inhibiting nuclear translocalization of STAT1 and NFAT‐1 in response to IFNβ, thus, indicating calreticulin downregulation linked with Type I IFN antagonism of JEV NS5 via activation of Ca(2+)/calicineurin. Calcineurin inhibitor cyclosporin A attenuated NS5‐mediated inhibition of IFNβ‐induced responses, for example, IFN‐sensitive response element driven luciferase, STAT1‐dependent PKR mRNA expression, as well as phosphorylation and nuclear translocation of STAT1. Transfection with calcineurin (vs. control) siRNA enhanced nuclear translocalization of STAT1 and upregulated PKR expression in NS5‐expressing cells in response to IFNβ. Results prove Ca(2+), calreticulin, and calcineurin involvement in STAT1‐mediated signaling as well as a key role of JEV NS5 in Type I IFN antagonism. This study offers insights into the molecular mechanism of Type I interferon antagonism by JEV NS5.
has issue date	2013-12-02(xsd:dateTime)
bibo:doi	10.1002/pmic.201300001
bibo:pmid	24166946
has license	no-cc
sha1sum (hex)	bc95b9ddd749ba23a66666276f284b0f0649bfbe
schema:url	https://doi.org/10.1002/pmic.201300001
resource representing a document's title	Proteomic analysis for Type I interferon antagonism of Japanese encephalitis virus NS5 protein
has PubMed Central identifier	PMC7167617
has PubMed identifier	24166946
schema:publication	Proteomics
resource representing a document's body	covid:bc95b9ddd749ba23a66666276f284b0f0649bfbe#body_text
is schema:about of	named entity 'cells' named entity 'antagonism' named entity 'Type I IFN' named entity 'antagonistic' named entity 'levels' named entity 'indicating' named entity 'ANTAGONISM' named entity 'RAISED' named entity 'CHARACTERIZES' named entity 'JAPANESE ENCEPHALITIS VIRUS' named entity 'PROTEOMIC ANALYSIS' named entity 'activation' named entity 'IFN' named entity 'Japanese encephalitis virus' named entity 'downregulation' named entity 'cyclosporin A' named entity 'responses' named entity 'mechanism' named entity 'PKR' named entity 'nonstructural protein' named entity 'study' named entity 'neuroblastoma' named entity 'nuclear' named entity 'response' named entity 'mRNA' named entity 'translocation' named entity 'silence' named entity 'This' named entity 'STAT1' named entity 'proteomic' named entity 'IFN' named entity 'STAT1' named entity 'mRNA expression' named entity 'calcineurin' named entity 'phosphoprotein' named entity 'STAT1' named entity 'calreticulin' named entity 'Type I interferon' named entity 'molecular mechanism' named entity 'IFN' named entity 'JEV' named entity 'Proteomic analysis' named entity 'Japanese encephalitis virus' named entity 'linked' named entity 'statistical analysis' named entity 'downregulated' named entity 'vector' named entity 'cyclophilin' named entity 'Microsoft Excel' named entity 'calreticulin' named entity 'protein expression' named entity 'actin' named entity 'post synaptic density' named entity 'nuclei' named entity 'PANTHER' named entity 'Transcriptional factor' named entity 'transfected' named entity 'calreticulin' named entity 'FITC' named entity 'phosphorylation' named entity 'vector' named entity 'IRF-3' named entity 'cyclophilin' named entity 'vector' named entity 'CsA' named entity 'antibody' named entity 'protein' named entity 'NS3' named entity 'multiplicity of infection' named entity 'ISRE' named entity 'vector' named entity 'medulloblastoma' named entity 'antibodies' named entity 'viruses'

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