About: Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry
Creator	Li, Feng Massire, Christian Sampath, Rangarajan Blyn, Lawrence Ecker, David Eshoo, Mark Hall, Thomas Hofstadler, Steven
Source	Medline; PMC
abstract	abstract: Newly emergent infectious diseases are a global public health problem. The population dense regions of Southeast Asia are the epicenter of many emerging diseases, as evidenced by the outbreak of Nipah, SARS, avian influenza (H5N1), Dengue, and enterovirus 71 in this region in the past decade. Rapid identification, epidemiologic surveillance, and mitigation of transmission are major challenges in ensuring public health safety. Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI‐MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. This approach is capable of automated analysis of more than 1,500 PCR reactions a day. It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens.
has issue date	2007-04-23(xsd:dateTime)
bibo:doi	10.1196/annals.1408.008
bibo:pmid	17470915
has license	no-cc
sha1sum (hex)	bc0b69d4de776320a5d83008c8b864e9161de3ae
schema:url	https://doi.org/10.1196/annals.1408.008
resource representing a document's title	Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry
has PubMed Central identifier	PMC7167958
has PubMed identifier	17470915
schema:publication	Ann N Y Acad Sci
resource representing a document's body	covid:bc0b69d4de776320a5d83008c8b864e9161de3ae#body_text
is schema:about of	named entity 'mass spectrometry' named entity 'disease surveillance' named entity 'regions' named entity 'chain reaction' named entity 'Identification' named entity 'PATHOGENS' named entity 'MITIGATION' named entity 'BACTERIAL' named entity 'MAJOR' named entity 'PROTOZOAL' named entity 'SARS' named entity 'BASE COMPOSITION' named entity 'IN THE PAST' named entity 'DESCRIBE' named entity 'MEASUREMENTS' named entity 'LARGE' named entity 'BASED' named entity 'TRANSMISSION' named entity 'VIRAL' named entity 'SURVEILLANCE' named entity 'PUBLIC HEALTH' named entity 'pathogens' named entity 'Southeast Asia' named entity 'products' named entity 'outbreak' named entity 'problem' named entity 'viral' named entity 'emerging' named entity 'Southeast Asia' named entity 'avian influenza' named entity 'enterovirus 71' named entity 'PCR' named entity 'public health' named entity 'PCR' named entity 'PCR' named entity 'influenza viruses' named entity 'Dengue' named entity 'PCR' named entity 'Americas' named entity 'SARS outbreak' named entity 'SARS CoV' named entity 'mass spectrometer' named entity 'PCR' named entity 'loci' named entity 'coronavirus' named entity 'SNPs' named entity 'coronavirus' named entity 'etiology' named entity 'alphaviruses' named entity 'alphaviruses' named entity 'pathogens' named entity 'ESI-MS' named entity 'primers' named entity 'RNA-dependent RNA polymerase' named entity 'infectious organism' named entity 'nucleic acid tests' named entity 'epidemics' named entity 'nucleotide' named entity 'viruses' named entity 'Coronaviridae' named entity 'amplicon' named entity 'microtiter plate' named entity 'Alphavirus' named entity 'search algorithm' named entity 'H9N2' named entity 'etiology' named entity 'PCR' named entity 'primer pairs' named entity 'viruses' named entity 'coronaviruses' named entity 'infectious agent' named entity 'viruses' named entity 'Viral genome' named entity 'serologically'

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