About: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation

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About: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
Creator	Wu, Ying Wang, Yanping Guo, Jianfeng Hao, Xiaojing Wang, Huaizhong Wang, Jiying
Source	PMC
abstract	Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the immune response of organism to RNA viruses. Quantitative real-time PCR (qRT-PCR) is a standard method for quantification of gene expression studies. However, the reliability of qRT-PCR data critically depends on proper selection of reference genes. In the study, using two different analysis programs, geNorm and NormFinder, we systematically evaluated the gene expression stability of six candidate reference genes (GAPDH, ACTB, B2M, RPL4, TBP, and PPIA) in samples of whole blood and PBMC with or without poly I:C stimulation. Generally, the six candidate genes performed a similar trend of expression stability in the samples of whole blood and PBMC, but more stably expressed in whole blood than in PBMC. geNorm ranked B2M and PPIA as the best combination for gene expression normalization, while according to NormFinder, TBP was ranked as the most stable reference gene, followed by B2M and PPIA. Comprehensively considering the results from the two programs, we recommended using the geometric mean of the three genes, TBP, PPIA and B2M, to normalize the gene expression of whole blood and PBMC with poly I:C stimulation. Our study is the first detailed survey of the gene expression stability in whole blood and PBMC with or without poly I:C stimulation and should be helpful for investigating the molecular mechanism involved in porcine whole blood and PBMC in response to poly I:C stimulation.
has issue date	2014-04-01(xsd:dateTime)
bibo:doi	10.5713/ajas.2013.13471
bibo:pmid	25049976
has license	cc-by-nc
sha1sum (hex)	bbfbefd19c9c471dbacabd1f57124d1ad36f24f5
schema:url	https://doi.org/10.5713/ajas.2013.13471
resource representing a document's title	Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
has PubMed Central identifier	PMC4093518
has PubMed identifier	25049976
schema:publication	Asian-Australas J Anim Sci
resource representing a document's body	covid:bbfbefd19c9c471dbacabd1f57124d1ad36f24f5#body_text
is schema:about of	named entity 'WHOLE BLOOD' named entity 'ANALYSIS' named entity 'BEST' named entity 'CANDIDATE' named entity 'RANKED' named entity 'RNA VIRUSES' named entity 'PBMC' named entity 'ACCORDING' named entity 'DETAILED' named entity 'REAL-TIME PCR ' named entity 'METHOD' named entity 'RESPONSE TO' named entity 'ACTB' named entity 'CANDIDATE GENES' named entity 'STIMULATION' named entity 'EXPRESSED' named entity 'PERFORMED' named entity 'BUT' named entity 'USING' named entity 'HELPFUL' named entity 'STUDIES' named entity 'IS A' named entity 'helpful' named entity 'gene' named entity 'stable' named entity 'PBMC' named entity 'reference' named entity 'stimulation' named entity 'expressed' named entity 'Peripheral' covid:arg/bbfbefd19c9c471dbacabd1f57124d1ad36f24f5 named entity 'PERIPHERAL BLOOD MONONUCLEAR CELLS' named entity 'PORCINE' named entity 'PCR DATA' named entity 'TREND' named entity 'IMMUNE RESPONSE' named entity 'PROGRAMS' named entity 'TBP' named entity 'PROPER' named entity 'STUDIES' named entity 'GENES' named entity 'WHOLE BLOOD' named entity 'GENE EXPRESSION' named entity 'ACID' named entity 'REFERENCE' named entity 'SELECTION' named entity 'PORCINE' named entity 'STABILITY' named entity 'INVOLVED' named entity 'GEOMETRIC MEAN' named entity 'NORMALIZE' named entity 'GENES' named entity 'PERIPHERAL BLOOD MONONUCLEAR CELLS' named entity 'SURVEY' named entity 'INVESTIGATING' named entity 'RPL4' named entity 'PPIA' named entity 'SELECTION' named entity 'DIFFERENT' named entity 'FOLLOWED BY' named entity 'STANDARD' named entity 'RECOMMENDED' named entity 'EXPRESSION' named entity 'RESULTS' named entity 'STABLE' named entity 'CONSIDERING' named entity 'REFERENCE GENE' named entity 'ORGANISM' named entity 'STIMULATION' named entity 'B2M' named entity 'RELIABILITY' named entity 'SIMILAR' named entity 'IMMUNE CELLS' named entity 'GENE EXPRESSION' named entity 'EVALUATED' named entity 'STUDY'

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