About: Modification of SARS-CoV S1 gene render expression in Pichia pastoris

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Modification of SARS-CoV S1 gene render expression in Pichia pastoris Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Modification of SARS-CoV S1 gene render expression in Pichia pastoris
Creator	Guo, Aizhen Lu, Haisong Ae, Chen Lu, H Tan, Yadi Ae, Yang Fei, Xiaozhan Guo, Ae Chen, Ae Ae, Guo Fei, Ae Guoliang, A Hongyan, A Huanchun, A Tan, Ae Tan, Y Yang, Ae
source	Medline; PMC
abstract	S1 gene fragment containing receptor-binding region was amplified by several sets of primers using Over-Lap PCR. The native S1 gene was modified at A + T abundant regions; n.t.777–1683, n.t.1041–1050, n.t.1236–1248, n.t.1317–1335, n.t.1590–1605; based on the same amino acid sequences. The modified gene was cloned into a yeast expression vector pPIC9K. The resultant plasmid pPIC9K- S1 was transformed into Pichia pastoris GS 115 and the protein expression was induced with methanol. SDS-PAGE confirmed that the recombinant SI was secreted in the supernatant of induced GS 115. The protein yield reached 69 mg/l. ELISA and Western blot demonstrated that the S1 could react with the convalescent sera of people infected by SARS-CoV. Furthermore, ligand blot assay showed that the recombinant S1 could react with ACE2, the receptor of SARS-CoV. The molecular mass of expressed S1 was about 70 kDa, which was higher than that of the 30 kDa expected. PNGase F deglycosylation resulted in a protein band of 30 kDa. In conclusion, the S1 gene modification rendered the high-level expression of S1 in P. pastoris GS 115 and the protein was secreted as a biologically active form which was hyperglycosylated.
has issue date	2006-01-01(xsd:dateTime)
bibo:doi	10.1007/s11262-006-0072-x
bibo:pmid	16991004
has license	no-cc
sha1sum (hex)	bbdf783c6c19786a7013646f1a6cddbc30fc9f6a
schema:url	https://doi.org/10.1007/s11262-006-0072-x
resource representing a document's title	Modification of SARS-CoV S1 gene render expression in Pichia pastoris
has PubMed Central identifier	PMC7088608
has PubMed identifier	16991004
schema:publication	Virus Genes
resource representing a document's body	covid:bbdf783c6c19786a7013646f1a6cddbc30fc9f6a#body_text
is schema:about of	named entity 'react' named entity 'kDa' named entity 'render' named entity 'PRIMERS' named entity 'GENE' named entity 'ASSAY' named entity 'SARS-COV' named entity '115' named entity '777' named entity '1050' named entity 'EXPRESSED' named entity 'PROTEIN BAND' named entity 'ELISA' named entity '1248' named entity 'MG%' named entity 'GENE MODIFICATION' named entity 'ACE2' covid:arg/bbdf783c6c19786a7013646f1a6cddbc30fc9f6a named entity 'SERA' named entity 'YEAST' named entity 'YIELD' named entity 'INFECTED' named entity 'TRANSFORMED' named entity 'EXPECTED' named entity 'SECRETED' named entity 'DEGLYCOSYLATION' named entity 'ACTIVE' named entity 'HIGH' named entity 'BIOLOGICALLY' named entity 'CONFIRMED' named entity 'PROTEIN ' named entity 'LEVEL' named entity 'LAP' named entity 'CONTAINING' named entity 'KDA' named entity 'SUPERNATANT' named entity 'HIGHER' named entity 'REGION' named entity 'PICHIA PASTORIS' named entity 'RECEPTORBINDING' named entity 'GENE FRAGMENT' named entity 'USING' named entity 'PLASMID' named entity 'RECEPTOR' named entity 'ABOUT' named entity 'ABUNDANT' named entity 'EXPRESSION' named entity 'A PROTEIN' named entity 'S1 GENE' named entity 'EXPRESSION VECTOR' named entity 'MODIFICATION' named entity 'RENDER' named entity 'PICHIA PASTORIS' named entity 'MODIFIED' named entity 'NATIVE' named entity 'BASED' named entity 'REACT' named entity 'SDS-PAGE' named entity '1590' named entity 'INDUCED' named entity 'CONCLUSION' named entity 'PEOPLE' named entity 'PCR' named entity 'AMPLIFIED' named entity 'METHANOL'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software