About: Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses
Creator	Foord, Adam White, John Heine, Hans Colling, Axel
Source	Medline; PMC
abstract	Microsphere suspension array systems enable the simultaneous fluorescent identification of multiple separate nucleotide targets in a single reaction. We have utilized commercially available oligo-tagged microspheres (Luminex MagPlex-TAG) to construct and evaluate multiplexed assays for the detection and differentiation of Hendra virus (HeV) and Nipah virus (NiV). Both these agents are bat-borne zoonotic paramyxoviruses of increasing concern for veterinary and human health. Assays were developed targeting multiple sites within the nucleoprotein (N) and phosphoprotein (P) encoding genes. The relative specificities and sensitivities of the assays were determined using reference isolates of each virus type, samples from experimentally infected horses, and archival veterinary diagnostic submissions. Results were assessed in direct comparison with an established qPCR. The microsphere array assays achieved unequivocal differentiation of HeV and NiV and the sensitivity of HeV detection was comparable to qPCR, indicating high analytical and diagnostic specificity and sensitivity.
has issue date	2013-02-06(xsd:dateTime)
bibo:doi	10.1155/2013/289295
bibo:pmid	23509705
has license	cc-by
sha1sum (hex)	baae5a4badccc0738ac8dc31e51c42c2da1afccc
schema:url	https://doi.org/10.1155/2013/289295
resource representing a document's title	Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses
has PubMed Central identifier	PMC3581118
has PubMed identifier	23509705
schema:publication	Biomed Res Int
resource representing a document's body	covid:baae5a4badccc0738ac8dc31e51c42c2da1afccc#body_text
is schema:about of	named entity 'Hendra virus' named entity 'NiV' named entity 'microsphere' named entity 'encoding' named entity 'NiV' named entity 'ARCHIVAL' named entity 'TAG' named entity 'BAT' named entity 'ISOLATES' named entity 'ENABLE' named entity 'SINGLE' named entity 'DIRECT' named entity 'GENES' named entity 'ASSESSED' named entity 'ZOONOTIC' named entity 'FLUORESCENT' named entity 'DETERMINED' named entity 'REACTION' named entity 'DIAGNOSTIC' covid:arg/baae5a4badccc0738ac8dc31e51c42c2da1afccc named entity 'detection' named entity 'HeV' named entity 'nucleoprotein' named entity 'separate' named entity 'samples' named entity 'suspension' named entity 'indicating' named entity 'Nipah virus' named entity 'identification' named entity 'veterinary' named entity 'multiplexed' named entity 'infected' named entity 'phosphoprotein' named entity 'virus' named entity 'Differentiation' named entity 'NiV' named entity 'virus' named entity 'Hendra virus' named entity 'human health' named entity 'microsphere' named entity 'HeV' named entity 'multiplexed' named entity 'zoonotic' named entity 'Viruses' named entity 'Differentiation' named entity 'Microsphere' named entity 'microsphere' named entity 'genome' named entity 'pathogens' named entity 'viruses' named entity 'ROC curve' named entity 'Malaysia' named entity 'microsphere' named entity 'NiV' named entity 'gene' named entity 'primers' named entity 'bat' named entity 'RNA' named entity 'RT-PCR' named entity 'qPCR' named entity 'microsphere' named entity 'zoonoses' named entity 'NiV' named entity 'genomic sequences' named entity 'microsphere' named entity 'hybridization' named entity 'henipavirus' named entity 'gene' named entity 'primers' named entity 'India' named entity 'multiplex assay' named entity 'Microsphere' named entity 'Bangladesh' named entity 'bat' named entity 'Hendra virus' named entity 'GenBank' named entity 'zoonoses' named entity 'epidemics'

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