About: Screening and Identification of a Chicken Dendritic Cell Binding Peptide by Using a Phage Display Library

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About: Screening and Identification of a Chicken Dendritic Cell Binding Peptide by Using a Phage Display Library Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Screening and Identification of a Chicken Dendritic Cell Binding Peptide by Using a Phage Display Library
Creator	Wang, Li Cui, Wen Jiang, Yanping Li, Yijing Qiao, Xinyuan Tang, Lijie Xu, Yigang Huang, Xuewei Ma, Sunting Wang, Xiaona Zhou, Han Dixon, Brian Berinstein, Analía Santander, Javier
Source	PMC
abstract	Dendritic cells (DCs), as antigen-presenting cells, can initiate adaptive immune responses efficiently. Although the DC-targeting strategy has attracted more attention, relevant studies on chicken are rare. Here, specific chicken bone marrow DC-binding peptides were selected using a phage display peptide library and confirmed through ELISA, flow cytometry, fluorescence microscopy, and laser confocal microscopy. The peptide candidate SPHLHTSSPWER, named SP, was fused to the infectious bursal disease virus (IBDV) structural protein and protective antigen VP2. In vitro, the expression of DC markers (CD80, CD83, CD86, DEC205, and MHCII) and some cytokines (IFN-γ, IL-12, TNF-α, IL-1β, IL-6, and CXCLi1) by VP2-SP-stimulated DCs was significantly higher than that by DCs treated with the VP2-control peptide at 4 h (p < 0.001). In addition, an oral vaccine targeting DCs was generated using chicken-borne Lactobacillus saerimneri M11 (L. sae M11) to deliver VP2 fused with SP. Anti-IBDV mucosal and humoral immune responses were induced efficiently via oral administration, resulting in higher protective efficacy in the VP2-SP group than the VP2 group. Therefore, chicken DC targeting of IBDV protective antigen VP2 delivered by L. sae provides effective immune protection in chicken. Our study may promote research on the DC-targeting strategy to enhance the effectiveness of chicken vaccines.
has issue date	2019-08-06(xsd:dateTime)
bibo:doi	10.3389/fimmu.2019.01853
bibo:pmid	31447851
has license	cc-by
sha1sum (hex)	b8b5d71242ee50c531cab2c04176241bc48fc468
schema:url	https://doi.org/10.3389/fimmu.2019.01853
resource representing a document's title	Screening and Identification of a Chicken Dendritic Cell Binding Peptide by Using a Phage Display Library
has PubMed Central identifier	PMC6691127
has PubMed identifier	31447851
schema:publication	Front Immunol
resource representing a document's body	covid:b8b5d71242ee50c531cab2c04176241bc48fc468#body_text
is schema:about of	named entity 'CELL BINDING' named entity 'CHICKEN' named entity 'CHICKEN BONE' named entity 'CHICKEN' named entity '0.001' named entity 'STUDY' named entity 'CONFIRMED' named entity 'DEC205' named entity 'USING' named entity 'STRUCTURAL PROTEIN' named entity 'LASER' named entity 'SELECTED' named entity 'FUSED TO' named entity 'STRATEGY' named entity 'VACCINES' named entity 'PROTECTIVE ANTIGEN ' named entity 'BORNE' named entity 'CD83' named entity 'HIGHER' named entity 'PEPTIDE' named entity 'TO DELIVER' named entity 'TARGETING' named entity 'ENHANCE' named entity 'PROTEIN ' named entity 'SAE' named entity 'PHAGE DISPLAY LIBRARY' named entity 'PEPTIDE' named entity 'DELIVERED' named entity 'DENDRITIC CELLS' named entity 'EFFECTIVENESS' named entity 'IN VITRO' named entity 'LACTOBACILLUS SAERIMNERI' named entity 'CONFOCAL MICROSCOPY' named entity 'ELISA' named entity 'OUR' named entity 'EXPRESSION' named entity 'BONE MARROW' named entity 'PHAGE DISPLAY PEPTIDE LIBRARY' named entity 'CANDIDATE' named entity 'RESEARCH' named entity 'RARE' named entity 'INDUCED' named entity 'IMMUNE PROTECTION' named entity 'PROVIDES' named entity 'INFECTIOUS BURSAL DISEASE VIRUS' named entity 'TREATED WITH' named entity 'BINDING' named entity 'ADDITION' named entity 'PROMOTE' named entity 'ANTIGEN-PRESENTING CELLS' named entity '28P' named entity 'INITIATE' named entity 'RESULTING IN' named entity 'RELEVANT' named entity 'GENERATED' named entity 'HERE' named entity 'FUSED WITH' named entity 'DCS' named entity 'MARKERS' named entity 'NAMED' named entity 'M11' named entity 'PEPTIDES' named entity 'FLUORESCENCE MICROSCOPY' named entity 'IL-1' named entity 'CONTROL' named entity 'CYTOKINES' named entity 'USING' named entity 'ORAL VACCINE'

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