About: The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells

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About: The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells
Creator	Wu, Xiao Shen, Xu Wang, Xiao Yang, Rui Yu, Hao Jiang, Wei Sun, Bing Li, Lin Lin, Ying Jiang, Hua Shang, Bo Tian, Lin Yang, Sheng Wu, Jia Pei, Gang Lin, Guo Dai, Er Gan, Xiao Ji, Yong Ma, Zhi Wu, Ya Xie, You Zhu, Xue
Source	Medline; PMC
abstract	Spike protein is one of the major structural proteins of severe acute respiratory syndrome-coronavirus. It is essential for the interaction of the virons with host cell receptors and subsequent fusion of the viral envelop with host cell membrane to allow infection. Some spike proteins of coronavirus, such as MHV, HCoV-OC43, AIBV and BcoV, are proteolytically cleaved into two subunits, S1 and S2. In contrast, TGV, FIPV and HCoV-229E are not. Many studies have shown that the cleavage of spike protein seriously affects its function. In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV, we generated S1 and S2 subunit specific antibodies (Abs) as well as N, E and 3CL protein-specific Abs. Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from E.coli expressed or lysate of SARS-CoV infected Vero-E6 cells by Western blot analysis. Furthermore, the anti-S1 and S2 Abs were proved to be capable of binding to SARS CoV under electron microscope observation. When S2 Ab was used to perform immune precipitation with lysate of SARS-CoV infected cells, a cleaved S2 fragment was detected with S2-specific mAb by Western blot analysis. The data demonstrated that the cleavage of S protein was observed in the lysate, indicating that proteolytic processing of S protein is present in host cells.
has issue date	2004-10-01(xsd:dateTime)
bibo:doi	10.1038/sj.cr.7290240
bibo:pmid	15450134
has license	no-cc
sha1sum (hex)	b84856fa2bf61e462f2cfe3545286469418ed8de
schema:url	https://doi.org/10.1038/sj.cr.7290240
resource representing a document's title	The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells
has PubMed Central identifier	PMC7091875
has PubMed identifier	15450134
schema:publication	Cell Res
resource representing a document's body	covid:b84856fa2bf61e462f2cfe3545286469418ed8de#body_text
is schema:about of	named entity 'protein' named entity 'cleaved' named entity 'maturation' named entity 'respiratory' named entity 'coronavirus' named entity 'E.coli' named entity 'host' named entity 'observed' named entity 'TGV' named entity 'virus' named entity 'PROTEIN ' named entity 'SPIKE' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'MATURATION' named entity 'RECEPTORS' named entity 'RESULTS' named entity 'IMMUNE PRECIPITATION' named entity 'CELLS' named entity 'ELECTRON MICROSCOPE' named entity 'PERFORM' named entity 'PROTEIN ' named entity 'MHV' named entity 'FIPV' named entity 'STUDIES' named entity 'ABS' named entity 'LYSATE' named entity 'GENERATED' named entity 'E.COLI' named entity 'DETECTED' named entity 'VERO' named entity 'VERO' named entity 'ORDER' named entity 'PROTEIN' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'CLEAVED' named entity 'INFECTED' named entity 'VIRUS' named entity 'SPIKE PROTEIN' named entity 'INFECTED' named entity 'SUBUNIT' named entity 'TO INVESTIGATE' named entity 'THEIR' named entity 'WESTERN BLOT ANALYSIS' named entity 'HCOV-229E' named entity 'SARS' named entity 'INDICATING' named entity 'SPECIFIC' named entity 'CLEAVAGE' named entity 'HOST CELL MEMBRANE' named entity 'ALLOW' named entity 'CONTRAST' named entity 'BIND' named entity 'FRAGMENT' named entity 'OBSERVATION' named entity 'ONE OF' named entity 'BINDING' named entity 'STRUCTURAL PROTEINS' named entity 'USED' named entity 'PROTEINS'

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