About: Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa
Creator	Klimenko, Natalia Tyakht, Alexander Alexeev, Dmitry Chuprikova, Liubov Filipenko, Maxim Koshechkin, Stanislav Kurina, Irina Popenko, Anna
Source	Elsevier; Medline; PMC
abstract	Abstract Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the “golden standard” for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks.
has issue date	2020-04-15(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2020.101570
bibo:pmid	32304824
has license	els-covid
sha1sum (hex)	b810e561131d85023f028d4c09da6209190f59ed
schema:url	https://doi.org/10.1016/j.mcp.2020.101570
resource representing a document's title	Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa
has PubMed Central identifier	PMC7158812
has PubMed identifier	32304824
schema:publication	Mol Cell Probes
resource representing a document's body	covid:b810e561131d85023f028d4c09da6209190f59ed#body_text
is schema:about of	named entity 'data' named entity 'human gut microbiome' named entity 'human gut' named entity 'clinical trials' named entity 'diet' named entity 'profiling' named entity 'AGE' named entity 'SAMPLES' named entity 'FORM' named entity 'QPCR' named entity 'PCR' named entity 'The platform' named entity 'prokaryotic' named entity 'high-throughput' named entity 'probes' named entity 'gut microbiome' named entity 'Bio-Rad' named entity 'oligonucleotide' named entity 'platform' named entity 'taxa' named entity 'conducting' named entity 'composition' named entity 'Development' named entity 'high-throughput' named entity 'clinical trials' named entity 'microbial' named entity 'qPCR' named entity 'qPCR' named entity 'glucose intolerance' named entity 'stool test' named entity 'phenotypes' named entity 'gene' named entity 'consensus sequences' named entity 'Clostridiales' named entity 'gut microbiome' named entity 'coefficient of determination' named entity 'Positive correlation' named entity 'type 2 diabetes' named entity 'gut microbial' named entity 'Bio-Rad' named entity 'primers' named entity 'amplicon' named entity 'immunocompromised patients' named entity 'metabolic disorders' named entity 'primers' named entity 'ulcerative colitis' named entity 'probiotic' named entity 'denaturation' named entity 'anti-inflammatory' named entity 'variance is explained' named entity 'taxa' named entity 'taxon' named entity '16S rRNA' named entity 'Rospotrebnadzor' named entity 'real-time PCR' named entity 'taxonomic' named entity 'microbiome' named entity '16 mM' named entity 'virulence factors' named entity 'IBD' named entity '16S rRNA' named entity '16 mM' named entity 'taxonomic' named entity 'IBD' named entity 'Skolkovo Foundation' named entity 'metagenomics' named entity 'Tween 20' named entity 'microbiome' named entity '16S rRNA gene sequencing' named entity 'probiotics' named entity 'qPCR' named entity 'microbiome' named entity 'phylum' named entity 'taxonomic classification'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software