About: Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies

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About: Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies
Creator	Enjuanes, Luis Risco, Cristina Bullido, Maria Langeveld, Jan Ant6n, Inrs Corsin, Marta Gonzfilez, Silvia
Source	Elsevier; Medline; PMC
abstract	Abstract Following infection of haplotype defined NIH-miniswine with virulent transmissible gastroenteritis coronavirus (TGEV), isolated mesenteric lymph node CD4+ T-cells mounted a specific proliferative response against infectious or inactivated purified virus in secondary in vitro stimulation. A specific, dose-dependent response to the three major recombinant viral proteins: spike (S), membrane (M), and nucleoprotein (N), purified by affinity chromatography, was characterized. Induction of in vitro antibody synthesis was analyzed. The purified recombinant viral proteins induced the in vitro synthesis of neutralizing TGEV-specific antibodies when porcine TGEV-immune cells were stimulated with each of the combinations made with two of the major structural proteins: S+N, S+M, and to a minor extent with M+N, but not by the individual proteins. S-protein was dissociated from purified virus using NP-40 detergent and then micellar S-protein oligomers (S-rosettes) were formed by removing the detergent. These occurred preferentially by the association of more than 10 S-protein trimmers. These S-rosettes in collaboration with either N or M-proteins elicited TGEV-specific antibodies with titers up to 84 and 60%, respectively, of those induced by the whole virus. N-protein could be partially substituted by a 15-mer peptide that represents a T helper epitope previously identified in N-protein (Antón et al. (1995)). These results indicate that the induction of high levels of TGEV-specific antibodies requires stimulation by at least two viral proteins, and that optimum responses are induced by a combination of S-rosettes and the nucleoprotein.
has issue date	1996-12-02(xsd:dateTime)
bibo:doi	10.1016/s0168-1702(96)01390-1
bibo:pmid	9029784
has license	els-covid
sha1sum (hex)	b65e0d8d4fe0bd539a64eeb883a7b76841570080
schema:url	https://doi.org/10.1016/s0168-1702%2896%2901390-1
resource representing a document's title	Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies
has PubMed Central identifier	PMC7134021
has PubMed identifier	9029784
schema:publication	Virus Research
resource representing a document's body	covid:b65e0d8d4fe0bd539a64eeb883a7b76841570080#body_text
is schema:about of	named entity 'IN VITRO' named entity 'COOPERATION' named entity 'viral' named entity 'proteins' named entity 'induced' named entity 'substituted' named entity '1995' named entity 'response' named entity 'neutralizing' named entity 'titers' named entity 'Cooperation' named entity 'DEFINED' named entity 'USING' named entity 'RESPONSE' named entity 'RESULTS' named entity 'EXTENT' named entity 'INFECTIOUS' named entity 'INACTIVATED' named entity 'MEMBRANE' named entity 'ELICITED' named entity 'OLIGOMERS' named entity 'DISSOCIATED FROM' named entity 'DOSE-DEPENDENT' named entity 'NUCLEOPROTEIN' named entity 'STRUCTURAL PROTEINS' named entity 'AFFINITY CHROMATOGRAPHY' named entity 'MORE THAN' named entity '60%' named entity 'COMBINATIONS' named entity 'VIRULENT' named entity 'CD4' named entity 'ANTIBODY' named entity 'DETERGENT' named entity 'COULD BE' named entity 'SPIKE' named entity 'CHARACTERIZED' named entity 'FORMED' named entity 'IDENTIFIED' named entity 'PURIFIED' named entity 'PEPTIDE' named entity 'FOLLOWING' named entity 'PARTIALLY' named entity 'N-PROTEIN' named entity 'PROTEINS' named entity 'TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS' named entity 'COLLABORATION' named entity 'OPTIMUM' named entity 'TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS' named entity 'ASSOCIATION' named entity 'PORCINE' named entity 'RESPONSE TO' named entity 'A MINOR' named entity 'EITHER' named entity 'T-CELLS' named entity 'RECOMBINANT' named entity '28S' named entity 'VIRUS' named entity 'VIRAL PROTEINS' named entity 'REMOVING' named entity 'STIMULATED' named entity 'INDIVIDUAL' named entity 'INDUCTION' named entity 'SECONDARY' named entity 'MOUNTED' named entity 'S-PROTEIN' named entity 'T HELPER' named entity 'MESENTERIC LYMPH NODE' named entity 'SPECIFIC' named entity 'UP TO' named entity 'STIMULATION' named entity 'A T' named entity 'SPECIFIC' named entity 'INDUCTION' named entity 'STRUCTURAL PROTEINS' named entity 'VIRUS'

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