About: Genotyping of human bocavirus using a restriction length polymorphism

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Genotyping of human bocavirus using a restriction length polymorphism
Creator	Schildgen, Oliver Ditt, Vanessa Ae, Schildgen Sergei, A Tillmann, Ramona Verena, A Ae, Viazov
Source	Medline; PMC
abstract	Sequencing analysis of the isolates of a recently identified pathogen associated with respiratory infections, human bocavirus (HBoV), allowed for identification of two virus genotypes of the virus. In the current article a new method for a simple and fast differentiation of HBoV genotypes in clinical materials is described. The test includes an amplification of a 309 bp fragment of VP1/VP2 gene of HBoV from nasopharyngeal aspirates with a subsequent incubation of a PCR mix with the BstAPI endonuclease. Upon such a digestion, the DNA fragment derived from the genotype I HBoV isolates forms two fragments of 150 and 159 bp, while that obtained from genotype 2 isolates remains unrestricted. The developed technique may be used in epidemiological studies of HBoV infection and analysis of the potential differences in biological characteristics of HboV genotypes.
has issue date	2007-12-11(xsd:dateTime)
bibo:doi	10.1007/s11262-007-0182-0
bibo:pmid	18071891
has license	no-cc
sha1sum (hex)	b5bb2a61bd4207c1b808b291fabab77d3793cd2c
schema:url	https://doi.org/10.1007/s11262-007-0182-0
resource representing a document's title	Genotyping of human bocavirus using a restriction length polymorphism
has PubMed Central identifier	PMC7089024
has PubMed identifier	18071891
schema:publication	Virus Genes
resource representing a document's body	covid:b5bb2a61bd4207c1b808b291fabab77d3793cd2c#body_text
is schema:about of	named entity 'gene' named entity 'METHOD' named entity 'DNA FRAGMENT' named entity 'MIX' named entity 'GENOTYPES' named entity 'MATERIALS' named entity 'BIOLOGICAL' named entity 'ANALYSIS' named entity 'EPIDEMIOLOGICAL STUDIES' named entity 'ASSOCIATED WITH' named entity 'fast' named entity 'isolates' named entity 'genotypes' named entity 'genotypes' named entity 'human bocavirus' named entity 'virus' named entity 'genotype' named entity 'human bocavirus' named entity 'genotype' named entity 'mix' named entity 'nucleotide' named entity 'genotype' named entity 'genotypes' named entity 'genotypes' named entity 'restriction endonuclease' named entity 'PCR' named entity 'genotyping' named entity 'primer' named entity 'primers' named entity 'gene' named entity 'respiratory disease' named entity 'primer' named entity 'England' named entity 'VP1' named entity 'assay' named entity 'genotype' named entity 'electrophoresis' named entity 'genotypes' named entity 'infection' named entity 'gene' named entity 'primers' named entity 'DNA analysis' named entity 'nucleotide' named entity 'highly conserved' named entity 'Genome' named entity 'A81' named entity 'VP1' named entity 'GenBank' named entity 'nucleotide' named entity 'genotype' named entity 'nasopharyngeal' named entity 'Stockholm' named entity 'Perkin Elmer' named entity 'lower respiratory tract' named entity 'coronaviruses' named entity 'primer' named entity 'New England BioLabs' named entity 'restriction site' named entity 'genotyping' named entity 'VP1' named entity 'QIAGEN' named entity 'gene' named entity 'restriction site' named entity 'conserved regions' named entity 'VP1' named entity 'genotype' named entity 'genotypes' named entity 'DNA sequencing' named entity 'phylogenetic analysis' named entity 'VP1' named entity 'Parvoviridae' named entity 'gene' named entity 'Roche' named entity 'genotypes' named entity 'staining'

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