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  • Further studies showed that silencing of the PERK gene with siRNA attenuated VacA - mediated phosphorylation of eIF2alpha , CHOP induction , expression of BH3 - only protein Bim and Bax activation , and cell death induced by VacA with ammonium chloride , indicating that ER stress may lead to mitochondrial dysfunction during VacA - induced toxicity .
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