About: Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus

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About: Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus
Creator	Ishida, Isao Kirikae, Teruo Sasazuki, Takehiko Fukushi, Masaya Hatakeyama, Seisuke Itoh, Kyogo Kanai, Takao Komatsu, Nobukazu Matsuoka, Yusuke Miyoshi-Akiyama, Tohru Shichijo, Shigeki Ueshiba, Hidehiro
Source	Elsevier; Medline; PMC
abstract	Abstract When expressed in mammalian cells, the nucleocapsid (N) and membrane (M) proteins of the severe acute respiratory syndrome coronavirus (SARS-CoV) are sufficient to form pseudoparticles. To identify region(s) of the N molecule required for pseudoparticle formation, we performed biochemical analysis of the interaction of N mutants and M in HEK293 cells. Using a peptide library derived from N, we found that amino acids 101–115 constituted a novel binding site for M. We examined the ability of N mutants to interact with M and form pseudoparticles, and our observations indicated that M bound to NΔ(101–115), N1–150, N151–300, and N301–422, but not to N1–150Δ(101–115). However, pseudoparticles were formed when NΔ(101–115) or N301–422, but not N1–150 or N151–300, were expressed with M in HEK293 cells. These results indicated that the minimum portion of N required for the interaction with M and pseudoparticle formation consists of amino acids 301–422.
has issue date	2008-10-10(xsd:dateTime)
bibo:doi	10.1016/j.virol.2008.07.012
bibo:pmid	18703211
has license	els-covid
sha1sum (hex)	af0891ea4c8c5d5bb900567f72030d448e4caddf
schema:url	https://doi.org/10.1016/j.virol.2008.07.012
resource representing a document's title	Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus
has PubMed Central identifier	PMC7103410
has PubMed identifier	18703211
schema:publication	Virology
resource representing a document's body	covid:af0891ea4c8c5d5bb900567f72030d448e4caddf#body_text
is schema:about of	named entity 'MEMBRANE' named entity 'amino acids' named entity 'TO FORM' named entity 'REQUIRED' named entity 'EXPRESSED' named entity 'NOVEL' named entity 'PORTION' named entity 'TO FORM' named entity 'USING' named entity 'BUT' named entity '150' named entity 'TO IDENTIFY' named entity 'EXAMINED' named entity '115' named entity 'MOLECULE' named entity 'REGION' named entity 'RESULTS' named entity 'DERIVED' named entity 'SUFFICIENT' named entity 'FOUND' named entity '300' named entity 'CELLS' named entity 'FORMATION' named entity 'IDENTIFICATION' named entity 'MINIMUM' named entity 'BOUND' named entity 'REGIONS' named entity 'NUCLEOCAPSID' named entity 'SARS CORONAVIRUS' named entity 'REQUIRED' named entity 'PERFORMED' named entity 'THESE' named entity 'TO INTERACT WITH' named entity '422' named entity 'PROTEINS' named entity 'DISSECTION' named entity 'INTERACTION' named entity 'OUR' named entity '28S' named entity '101' named entity 'MEMBRANE' named entity 'ABILITY' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'BINDING SITE' named entity 'FORM' named entity 'NUCLEOCAPSID' named entity 'MUTANTS' named entity 'PEPTIDE LIBRARY' named entity 'HEK293 CELLS' named entity 'PROTEINS' named entity 'INDICATED' named entity 'INTERACTION' named entity 'FORMED' named entity 'BIOCHEMICAL ANALYSIS' named entity 'OBSERVATIONS' named entity 'AMINO ACIDS' named entity 'MAMMALIAN' named entity 'bound' named entity 'When' named entity 'membrane' named entity 'biochemical analysis' named entity 'severe acute respiratory syndrome coronavirus' named entity 'nucleocapsid' named entity 'amino acids' named entity 'amino acids' named entity 'pseudoparticle' named entity 'membrane (M) proteins' named entity 'nucleocapsid' named entity 'nucleocapsid' named entity 'Coronaviruses'

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