About: An LC-MRM assay for the quantification of metanephrines from dried blood spots for the diagnosis of pheochromocytomas and paragangliomas

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About: An LC-MRM assay for the quantification of metanephrines from dried blood spots for the diagnosis of pheochromocytomas and paragangliomas Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An LC-MRM assay for the quantification of metanephrines from dried blood spots for the diagnosis of pheochromocytomas and paragangliomas
Creator	Richard, Vincent Borchers, Christoph Eintracht, Shaun Zahedi, René
Source	Elsevier; Medline; PMC
abstract	The quantitation of metanephrine (MN), normetanephrine (NMN), and 3-methoxytyramine (3MT) – referred to as metanephrines -- by LC-MS/MS is the gold-standard for screening for pheochromocytoma and paragangliomas (PPGLs), tumors of the adrenal gland and the peripheral nervous system. An assay for metanephrines from dried blood spots (DBSs) would be of high clinical utility as it simplifies sample collection, enables remote sampling, and could increase compliance with the clinical recommendation for supine sampling. Moreover, DBS sampling facilitates the measurement of blood-derived metanephrines in pediatric patients – where DBSs are well-established – in order to diagnose neuroblastomas. Here, we adapted an established derivatization-based LC-MRM-MS assay for plasma catecholamines, and optimized the sample extraction, LC, and MS parameters to produce a fast, sensitive, and robust method for the measurement of metanephrines from DBSs, including 3-methoxytyramine. The DBS samples were excised, derivatized with phenyl isothiocyanate (PITC) on-spot, extracted, and measured by LC-MRM-MS. To validate assay suitability and performance, we assessed the linearity, precision, accuracy, recovery, and matrix effects of the method, and determined the stability of metanephrines in DBSs under different storage conditions. Assay performance for NMN, MN, and 3MT was sufficient for quantitation from a single DBS within a linear range from 40 – 2000 pg/mL MN and NMN were stable in DBSs for 2 weeks, whereas 3MT was stable for one week regardless of storage temperature. Altogether, this work represents the first quantitative LC-MS/MS method for metanephrines from DBSs and provides a novel opportunity for the diagnosis of PPGLs and neuroblastomas in the future.
has issue date	2020-07-09(xsd:dateTime)
bibo:doi	10.1016/j.aca.2020.06.020
bibo:pmid	32825898
has license	no-cc
sha1sum (hex)	ae7e9136d2141f225d63f4ac79a6a0274d79a26b
schema:url	https://doi.org/10.1016/j.aca.2020.06.020
resource representing a document's title	An LC-MRM assay for the quantification of metanephrines from dried blood spots for the diagnosis of pheochromocytomas and paragangliomas
has PubMed Central identifier	PMC7346842
has PubMed identifier	32825898
schema:publication	Anal Chim Acta
resource representing a document's body	covid:ae7e9136d2141f225d63f4ac79a6a0274d79a26b#body_text
is schema:about of	named entity 'diagnosis' named entity 'HPLC' named entity 'metanephrines' named entity 'dopamine' named entity 'mass spectrometer' named entity 'ion' named entity 'urine' named entity 'clinical guidelines' named entity 'centrifugation' named entity 'solid phase extraction' named entity 'metabolic' named entity 'epinephrine' named entity 'Mobile phase' named entity 'matrix effects' named entity 'LC-MS' named entity '3-methoxytyramine' named entity 'capillary' named entity 'catecholamines' named entity 'Agilent' named entity 'paragangliomas' named entity 'norepinephrine' named entity 'newborn screening' named entity 'urine' named entity 'metanephrines' named entity 'concentration level' named entity 'ion' named entity 'urine samples' named entity 'aqueous' named entity 'normetanephrine' named entity 'analyte' named entity 'metanephrines' named entity 'matrix effects' named entity 'precision, and accuracy' named entity 'calibration curve' named entity 'NMN' named entity 'NMN' named entity 'plasma' named entity 'MS/MS' named entity 'derivatized' named entity 'venous blood' named entity 'supine position' named entity 'ion' named entity 'follow-up' named entity 'NMN' named entity 'metanephrine' named entity 'Itis' named entity 'medulla' named entity 'ion' named entity 'metabolites' named entity 'metabolites' named entity 'plasma' named entity 'deuterium' named entity 'neuroblastoma' named entity 'metabolites' named entity 'accuracy and precision' named entity 'metabolites' named entity 'molecular weight cut-off' named entity 'Urine' named entity 'NMN' named entity 'Chromatographic separation' named entity 'biomarker' named entity 'matrix effects' named entity 'first-line' named entity 'biofluids' named entity 'adrenal' named entity 'derivatization' named entity 'reversed-phase chromatography' named entity 'formic acid' named entity 'ZipLoc' named entity 'Pheochromocytomas' named entity 'analyte' named entity 'catecholamines' named entity 'adrenal' named entity 'CT-scan' named entity 'NMN' named entity '3, 4' named entity 'plasma' named entity 'positive ion'

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