About: Monocytes and B cells support active replication of Chandipura virus

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About: Monocytes and B cells support active replication of Chandipura virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Monocytes and B cells support active replication of Chandipura virus
Creator	Gunjikar, Rashmi Pavitrakar, Daya Roy, Soumen Ayachit, Vijay Bondre, Vijay Sapkal, Gajanan
Source	Medline; PMC
abstract	BACKGROUND: Interaction between immune system and Chandipura virus (CHPV) during different stages of its life cycle remain poorly understood. The exact route of virus entry into the blood and CNS invasion has not been clearly defined. The present study was undertaken to assess the population in PBMC that supports the growth of virus and to detect active virus replication in PBMC as well as its subsets. METHODS: PBMC subsets viz.: CD3(+), CD14(+), CD19(+), CD56(+)cells were separated and infected with CHPV. The infected cells were then assessed for transcription (N gene primer) and replication (NP gene primer) of CHPV by PCR. The supernatant collected from infected cells were titrated in Baby Hamster Kidney (BHK) cells to assess virus release. The cytokine and chemokine expression was quantified by flow cytometry. RESULTS: Amplification of N and NP gene was detected in CD14(+) (monocyte) and CD19(+) (B cell), significant increase in virus titre was also observed in these subsets. It was observed that, although the levels of IL-6 and IL-10 were elevated in CD14(+) cells as compared to CD19(+)cells, the differences were not significant. However the levels of TNFα and IL-8 were significantly elevated in CD14(+) cells than in CD19(+)cells. The levels of chemokine (CXCL9, CCL5, CCL2, CXCL10) were significantly elevated in CHPV infected PBMC as compared to uninfected cells. CCL2 and CXCL9 were significantly increased in CHPV infected CD14(+)cells as compared to CD19(+) cells. CONCLUSION: CD14(+)and CD19(+)cells support active replication of CHPV. High viral load was detected in CD14(+) cells infected with CHPV hence it might be the primary target cells for active replication of CHPV. An elevated levels of cytokines and chemokines observed in CD14(+) cells may help in predicting the pathogenecity of CHPV and possible entry into the central nervous system.
has issue date	2016-09-14(xsd:dateTime)
bibo:doi	10.1186/s12879-016-1794-6
bibo:pmid	27628855
has license	cc-by
sha1sum (hex)	acb8ae819eaf658a9e9704220f1e987a0365675e
schema:url	https://doi.org/10.1186/s12879-016-1794-6
resource representing a document's title	Monocytes and B cells support active replication of Chandipura virus
has PubMed Central identifier	PMC5024506
has PubMed identifier	27628855
schema:publication	BMC Infect Dis
resource representing a document's body	covid:acb8ae819eaf658a9e9704220f1e987a0365675e#body_text
is schema:about of	named entity 'cells' named entity 'virus' named entity 'monocyte' named entity 'active' named entity 'remain' named entity 'Background' named entity 'Monocytes' named entity 'SUPPORT' named entity 'TO DETECT' named entity 'ACTIVE' named entity 'LIFE CYCLE' named entity 'TRANSCRIPTION' named entity 'ELEVATED' named entity 'SEPARATED' named entity 'LEVELS' named entity 'VIRUS ENTRY' named entity 'ITS' named entity 'BACKGROUND' named entity 'IL-10' named entity 'VIRUS REPLICATION' named entity 'CYTOKINE ' named entity 'NOT SIGNIFICANT' covid:arg/acb8ae819eaf658a9e9704220f1e987a0365675e named entity 'BLOOD' named entity 'PREDICTING' named entity 'IL-6' named entity 'CHANDIPURA VIRUS' named entity 'DEFINED' named entity 'IMMUNE SYSTEM' named entity 'KIDNEY' named entity 'POPULATION' named entity 'GENE' named entity 'SUPERNATANT' named entity 'INCREASED' named entity 'CNS' named entity 'HIGH' named entity 'BABY' named entity 'EXPRESSION' named entity 'GROWTH' named entity 'UNINFECTED' named entity 'TARGET CELLS' named entity 'VIRAL LOAD' named entity 'RESULTS' named entity 'REPLICATION' named entity 'CHANDIPURA VIRUS' named entity 'MONOCYTES' named entity 'OBSERVED' named entity 'PCR' named entity 'INTERACTION' named entity 'EXACT' named entity 'CHEMOKINES' named entity 'ASSESS' named entity 'PRIMARY' named entity 'DETECTED' named entity 'METHODS' named entity 'INVASION' named entity 'CHEMOKINE' named entity 'FLOW CYTOMETRY' named entity 'SIGNIFICANT' named entity 'CD3' named entity 'CELLS' named entity 'CONCLUSION' named entity 'COMPARED' named entity 'STAGES' named entity 'IL-8' named entity 'PRIMER' named entity 'TITRE' named entity 'MIGHT BE' named entity 'CXCL9' named entity 'AMPLIFICATION' named entity 'CELL' named entity 'HAMSTER' named entity 'CXCL10' named entity 'CD19' named entity 'TNF' named entity 'CCL5'

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