About: The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel

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About: The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel
Creator	Pham, Thieng Delcour, Anne Dosey, Timothy Hyser, Joseph Perry, Jacob
Source	Medline; PMC
abstract	Viroporins are small virus-encoded ion channel proteins. Most viroporins are monovalent selective cation channels, with few showing the ability to conduct divalent cations, like calcium (Ca(2+)). Nevertheless, some viroporins are known to disrupt host cell Ca(2+) homeostasis, which is critical for virus replication and pathogenesis. Rotavirus nonstructural protein 4 (NSP4) is an endoplasmic reticulum transmembrane glycoprotein that has a viroporin domain (VPD), and NSP4 viroporin activity elevates cytosolic Ca(2+) in mammalian cells. The goal of this study was to demonstrate that the NSP4 VPD forms an ion channel and determine whether the channel can conduct Ca(2+). Using planar lipid bilayer and liposome patch clamp electrophysiology, we show that a synthetic peptide of the NSP4 VPD has ion channel activity. The NSP4 VPD was selective for cations over anions and channel activity was observed to have both well-defined “square top” openings as well as fast current fluctuations, similar to other viroporins. Importantly, the NSP4 VPD showed similar conductance of divalent cations (Ca(2+) and Ba(2+)) as monovalent cations (K(+)), but a viroporin defective mutant lacked Ca(2+) conductivity. These data demonstrate that the NSP4 VPD is a Ca(2+)-conducting viroporin and establish the mechanism by which NSP4 disturbs host cell Ca(2+) homeostasis.
has issue date	2017-03-03(xsd:dateTime)
bibo:doi	10.1038/srep43487
bibo:pmid	28256607
has license	cc-by
sha1sum (hex)	a81f08700b297b78c8f255c4a428f2675ca45ef9
schema:url	https://doi.org/10.1038/srep43487
resource representing a document's title	The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel
has PubMed Central identifier	PMC5335360
has PubMed identifier	28256607
schema:publication	Sci Rep
resource representing a document's body	covid:a81f08700b297b78c8f255c4a428f2675ca45ef9#body_text
is schema:about of	named entity 'cations' named entity 'monovalent' named entity 'current' named entity 'conduct' named entity 'pathogenesis' named entity 'proteins' named entity 'Most' named entity 'conductance' named entity 'OPEN' covid:arg/a81f08700b297b78c8f255c4a428f2675ca45ef9 named entity 'CATION CHANNELS' named entity 'OPENINGS' named entity 'PROTEIN ' named entity 'USING' named entity 'DOMAIN' named entity 'IS A' named entity 'VIRUS REPLICATION' named entity 'ELECTROPHYSIOLOGY' named entity 'LIKE' named entity 'ION CHANNEL' named entity 'VPD' named entity 'FORMS' named entity 'CELLS' named entity 'NSP4' named entity 'PATCH CLAMP' named entity 'SELECTIVE' named entity 'FAST' named entity 'DETERMINE' named entity 'BUT' named entity 'VIRUS' named entity 'MUTANT' named entity 'HOMEOSTASIS' named entity 'OBSERVED' named entity 'MAMMALIAN' named entity 'LIPOSOME' named entity 'PROTEINS' named entity 'ION CHANNEL ACTIVITY' named entity 'CONDUCTANCE' named entity 'MONOVALENT CATIONS' named entity 'HAVE' named entity 'CATIONS' named entity 'CRITICAL' named entity 'DATA' named entity 'DOMAIN' named entity 'CALCIUM' named entity 'WELL-DEFINED' named entity 'THESE' named entity 'ENCODED' named entity 'VIROPORIN' named entity 'CHANNEL' named entity 'ENDOPLASMIC RETICULUM' named entity 'CURRENT' named entity 'VIROPORIN' named entity 'NSP4' named entity 'OPEN' named entity 'PATHOGENESIS' named entity 'STUDY' named entity 'MECHANISM' named entity 'SYNTHETIC PEPTIDE' named entity 'ELEVATES' named entity 'ANIONS' named entity 'ROTAVIRUS' named entity 'ESTABLISH' named entity 'DIVALENT CATIONS' named entity 'SMALL' named entity 'HOST CELL' named entity 'PLANAR' named entity 'FLUCTUATIONS' named entity 'CALCIUM' named entity 'SIMILAR' named entity 'GOAL' named entity 'ROTAVIRUS' named entity 'CONDUCTING' named entity 'CONDUCT' named entity 'ACTIVITY' named entity 'CONDUCTIVITY' named entity 'LIPID BILAYER'

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