About: Characteristics of seminal plasma proteins and their correlation with canine semen analysis

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Characteristics of seminal plasma proteins and their correlation with canine semen analysis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characteristics of seminal plasma proteins and their correlation with canine semen analysis
Creator	Barreto, C De Souza, F Lopes, M
Source	Elsevier; Medline; PMC
abstract	Abstract The objectives were to separate canine seminal plasma proteins (with SDS-PAGE) and to determine the correlation between specific proteins and semen characteristics. Three ejaculates from 20 mixed-breed dogs, of unknown fertility, were collected by digital manipulation. Ejaculate volume and color, sperm motility, sperm vigor, percentage of morphologically normal spermatozoa, and membrane integrity (hypoosmotic swelling test and fluorescent staining) were assessed. For each dog, seminal plasma was pooled from all three ejaculates and proteins were separated with SDS-PAGE, using polyacrylamide concentrations of 13% and 22% in the separation gels. After staining, gel images were digitized to estimate molecular weights (MW) and integrated optical density (IOD) of each lane and of individual bands. Total seminal plasma protein concentration was 2.19±1.56g/dL (mean±SD; range 1.12–5.19g/dL). A total of 37 protein bands were identified (although no dog had all 37 bands). In the 13% gel, molecular weights ranged from 100.6 to 17.1kDa, with four bands (49.7, 33.2, 26.4, and 19.5kDa) present in samples from all dogs. In the 22% gel, molecular weights ranged from 15.6 to 3.6kDa, with nine bands (15.6, 13.5, 12.7, 11.7, 10.5, 8.7, 7.8, 5.6, and 4.9kDa) present in samples from all dogs. Combined for both gels, the majority of bands (85%) had molecular weights <17kDa, with B20 (15.6kDa) in high concentrations in samples from all dogs. There were positive correlations (P ≤0.01) between two bands, B4 (67kDa) and B5 (58.6kDa), and sperm motility (r =0.66 and r =0.46), sperm vigor (r =0.56 and r =0.66), percentage of morphologically normal spermatozoa (r =0.55 and r =0.59), the hypoosmotic swelling test (r =0.76 and r =0.68), and fluorescent staining (r =0.56 and r =0.59), respectively. In conclusion, 37 proteins were identified in seminal plasma; two were significantly correlated with semen characteristics.
has issue date	2007-07-01(xsd:dateTime)
bibo:doi	10.1016/j.theriogenology.2006.11.016
bibo:pmid	17512978
has license	els-covid
sha1sum (hex)	a5aa231c68e922d1f7a450311b4618eb5ebe5109
schema:url	https://doi.org/10.1016/j.theriogenology.2006.11.016
resource representing a document's title	Characteristics of seminal plasma proteins and their correlation with canine semen analysis
has PubMed Central identifier	PMC7126591
has PubMed identifier	17512978
schema:publication	Theriogenology
resource representing a document's body	covid:a5aa231c68e922d1f7a450311b4618eb5ebe5109#body_text
is schema:about of	named entity 'PERCENTAGE' named entity 'COLOR' named entity 'PROTEIN ' named entity '28P' named entity 'SPECIFIC' named entity 'COMBINED' named entity 'INTEGRITY' named entity '0.59' named entity 'INDIVIDUAL' named entity 'DOG' named entity '0.01' named entity 'COLLECTED BY' named entity 'OBJECTIVES' named entity '2849' named entity 'HIGH' named entity '10.5' named entity 'PROTEINS' named entity 'sperm motility' named entity 'bands' named entity 'semen' named entity 'collected' named entity 'vigor' named entity 'kDa' named entity 'kDa' named entity 'bands' named entity 'For' named entity 'majority' named entity 'identified' named entity 'dogs' named entity 'digitized' named entity 'proteins' named entity 'CORRELATION' covid:arg/a5aa231c68e922d1f7a450311b4618eb5ebe5109 named entity 'GEL' named entity 'CONCLUSION' named entity 'DETERMINE' named entity 'MAJORITY' named entity 'THEIR' named entity 'SEMINAL PLASMA PROTEINS' named entity 'SEMINAL PLASMA PROTEINS' named entity 'SEMINAL PLASMA' named entity '11.7' named entity 'MANIPULATION' named entity 'SEPARATION' named entity 'NORMAL SPERMATOZOA' named entity 'USING' named entity '12.7' named entity 'AE 1' named entity 'DIGITAL' named entity 'TO SEPARATE' named entity 'FERTILITY' named entity 'IDENTIFIED' named entity 'STAINING' named entity 'PRESENT' named entity 'SDS-PAGE' named entity 'CHARACTERISTICS' named entity '2.19' named entity 'SEMEN ANALYSIS' named entity 'CHARACTERISTICS' named entity 'CANINE' named entity 'CORRELATED' named entity 'CANINE' named entity 'BREED' named entity 'TOTAL' named entity 'FLUORESCENT' named entity 'PROTEIN CONCENTRATION' named entity 'UNKNOWN' named entity 'SEPARATED' named entity 'INTEGRATED' named entity 'RANGE' named entity 'POLYACRYLAMIDE' named entity '19.5' named entity 'LANE' named entity '0.46' named entity 'EJACULATE VOLUME' named entity 'KDA' named entity 'ASSESSED' named entity 'MEMBRANE' named entity 'POOLED'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software