About: Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides

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About: Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides
Creator	Nitsche, Andreas Doellinger, Joerg Bartel, Sebastian Bourquain, Daniel Buchholz, Rainer Darsow, Kai Lange, Harald
Source	Medline; PMC
abstract	BACKGROUND: Despite the successful eradication of smallpox by the WHO-led vaccination programme, pox virus infections remain a considerable health threat. The possible use of smallpox as a bioterrorism agent as well as the continuous occurrence of zoonotic pox virus infections document the relevance to deepen the understanding for virus host interactions. Since the permissiveness of pox infections is independent of hosts surface receptors, but correlates with the ability of the virus to infiltrate the antiviral host response, it directly depends on the hosts proteome set. In this report the proteome of HEK293 cells infected with Vaccinia Virus strain IHD-W was analyzed by 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS in a bottom-up approach. RESULTS: The cellular and viral proteomes of VACV IHD-W infected HEK293 cells, UV-inactivated VACV IHD-W-treated as well as non-infected cells were compared. Derivatization of peptides with 4-sulfophenyl isothiocyanate (SPITC) carried out on ZipTipμ-C18 columns enabled protein identification via the peptides' primary sequence, providing improved s/n ratios as well as signal intensities of the PSD spectra. The expression of more than 24 human proteins was modulated by the viral infection. Effects of UV-inactivated and infectious viruses on the hosts' proteome concerning energy metabolism and proteins associated with gene expression and protein-biosynthesis were quite similar. These effects might therefore be attributed to virus entry and virion proteins. However, the modulation of proteins involved in apoptosis was clearly correlated to infectious viruses. CONCLUSIONS: The proteome analysis of infected cells provides insight into apoptosis modulation, regulation of cellular gene expression and the regulation of energy metabolism. The confidence of protein identifications was clearly improved by the peptides' derivatization with SPITC on a solid phase support. Some of the identified proteins have not been described in the context of poxvirus infections before and need to be further characterised to identify their meaning for apoptosis modulation and pathogenesis.
has issue date	2011-08-01(xsd:dateTime)
bibo:doi	10.1186/1743-422x-8-380
bibo:pmid	21806805
has license	cc-by
sha1sum (hex)	a27cb38e8670f317f707a16661115a42b5b1b9fe
schema:url	https://doi.org/10.1186/1743-422x-8-380
resource representing a document's title	Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides
has PubMed Central identifier	PMC3169512
has PubMed identifier	21806805
schema:publication	Virol J
resource representing a document's body	covid:a27cb38e8670f317f707a16661115a42b5b1b9fe#body_text
is schema:about of	named entity 'surface' named entity 'peptides' named entity 'MALDI' named entity 'GEL ELECTROPHORESIS' named entity 'IHD' named entity 'INFECTIONS' named entity 'AGENT' named entity 'MALDI' named entity 'ANALYZED' named entity 'REPORT' named entity 'PSD' named entity 'PSD' named entity 'PEPTIDES' named entity 'SUPPORT' named entity 'LED' named entity 'POSSIBLE' named entity 'HOSTS' named entity 'SUCCESSFUL' named entity 'PROGRAMME' named entity 'TOF MS' named entity 'PERMISSIVENESS' named entity 'THREAT' named entity 'DIRECTLY' named entity 'HEK 293 CELLS' named entity 'INFECTED' named entity 'TOF MS' named entity 'GEL ELECTROPHORESIS' named entity 'USE OF' named entity 'BUT' named entity 'INDEPENDENT OF' named entity 'BACKGROUND' named entity 'PROTEOME' named entity 'IHD' named entity 'PROTEOME ANALYSIS' named entity 'VACCINIA VIRUS' named entity 'SOLID PHASE' named entity '2-DIMENSIONAL' named entity 'DOCUMENT ' named entity 'VACCINATION' named entity 'RECEPTORS' named entity 'CONTINUOUS' named entity 'ANTIVIRAL' named entity 'RELEVANCE' named entity 'HEK293 CELLS' named entity 'ERADICATION' named entity 'CONSIDERABLE' named entity 'ZOONOTIC' named entity 'VIRUS HOST' named entity 'INFILTRATE' named entity 'APPROACH' named entity 'SURFACE' named entity 'INTERACTIONS' named entity 'UNDERSTANDING' named entity 'DEEPEN' named entity 'SET' named entity 'STRAIN' named entity 'POX VIRUS' named entity 'OCCURRENCE' named entity 'SMALLPOX' named entity '2-DIMENSIONAL' named entity 'POX' named entity 'VACCINIA VIRUS' named entity 'SULFONATED' named entity 'HOST RESPONSE' named entity 'INFECTED' named entity 'ABILITY' named entity 'VIRUS' named entity 'HEALTH' named entity 'BIOTERRORISM' covid:arg/a27cb38e8670f317f707a16661115a42b5b1b9fe named entity 'proteome' named entity 'infiltrate' named entity 'programme' named entity 'strain' named entity 'virus'

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