About: DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes

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About: DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
Creator	Costa-García, Agustín de la Escosura-Muñiz, Alfredo González-García, María
topic	covid:PMC7126624#this
Source	Elsevier; Medline; PMC
abstract	In this work, a gold complex is used as electroactive label for monitoring hybridization assays on glassy carbon electrodes. Ionic gold is bound to a 30-mer sequence of the SARS (severe acute respiratory syndrome) virus, responsible for the atypical pneumonia, using sodium aurothiomalate. In order to label this single strand, a mixture of sodium aurothiomalate and the strand is prepared. Then, it is incubated for 24 h at 37 °C and, finally, free gold is separated from the labeled strand by a dialysis against a 0.15 M NaCl solution (pH 7.5). The DNA hybridization sensor is designed immobilizing the complementary probe on the pre-treated electrode surface and, then, the hybridization reaction takes place with the gold labeled strand. The electrochemical determination is based on the catalytic effect of electrodeposited gold on the reduction of silver ions. In non-stringent experimental conditions, a limit of detection of 15 fmol (30 μL) is obtained, and discrimination between a complementary oligonucleotide and a three-based mismatch complementary oligonucleotide is achieved. For the discrimination of a single-base mismatch, is needed to use stringent conditions (50% of formamide in the hybridization buffer).
has issue date	2007-01-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2006.04.024
bibo:pmid	16762539
has license	no-cc
schema:url	https://doi.org/10.1016/j.bios.2006.04.024
resource representing a document's title	DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
has PubMed Central identifier	PMC7126624
has PubMed identifier	16762539
schema:publication	Biosens Bioelectron
resource representing a document's body	covid:PMC7126624#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/PMC7126624
is schema:about of	named entity 'DNA hybridization' named entity 'hybridization' named entity 'HCl' named entity 'limit of detection' named entity 'oxidized' named entity 'BSA' named entity 'complementary strand' named entity 'immunosensor' named entity 'complementary strand' named entity 'transition metal complexes' named entity 'Quake' named entity 'Eurogentec' named entity 'electrode' named entity 'biotin' named entity 'single-stranded' named entity 'SARS virus' named entity 'pure water' named entity 'reference electrode' named entity 'metal complexes' named entity 'adsorption' named entity 'oligonucleotide' named entity 'electrochemical' named entity 'correlation coefficient' named entity 'chemiluminescent' named entity 'silica' named entity 'biosensors' named entity 'adsorption' named entity 'oligonucleotide' named entity 'adsorption' named entity 'thiol group' named entity 'Spain' named entity 'pH 7' named entity 'circular dichroism' named entity 'electrodes' named entity 'oligonucleotide' named entity 'dialysed' named entity 'oligonucleotides' named entity '7.2' named entity 'single-base' named entity 'acoustic wave' named entity 'complementary strand' named entity 'GCEs' named entity 'nanoparticles' named entity 'oligonucleotide' named entity 'dialysis' named entity 'electrode' named entity 'fabrication technology' named entity 'electrode' named entity 'double strands' named entity 'electrodes' named entity 'silver nitrate' named entity 'glassy carbon' named entity 'Milli-Q' named entity 'immunoassays' named entity 'sodium aurothiomalate' named entity 'Spain' named entity 'HCl' named entity 'electrodeposited' named entity 'oligonucleotide' named entity 'fmol' named entity 'polymers' named entity 'thiol group' named entity 'electrochemical' named entity 'Non-covalent' named entity 'nmol' named entity 'sodium citrate' named entity 'single-base' named entity 'GCEs' named entity 'immunoglobulin' named entity 'colloidal gold' named entity 'impedance' named entity 'complementary strand' named entity 'hybridization' named entity 'hybridization' named entity 'sodium aurothiomalate' named entity 'Electrostatic' named entity 'sodium aurothiomalate' named entity 'biosensor'

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