About: covid:PMC7109869

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An Entity of Type : owl:Thing, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
Creator	Shirouzu, Mikako Takemoto, Chie Hanada, Kazuharu Katsura, Kazushige Matsuda, Takayoshi Ohsawa, Noboru Sakamoto, Kensaku Tomabechi, Yuri Yonemochi, Mayumi
is schema:about of	named entity 'cognate' named entity 'phosphoenolpyruvate' named entity 'ampicillin' named entity 'culture medium' named entity 'ultrafiltration' named entity 'NaCl' named entity 'S30' named entity 'alkaline' named entity 'NaCl' named entity 'recombinant' named entity 'alkaline' named entity 'cell-free' named entity 'T7 RNA polymerase' named entity 'NaCl' named entity 'GST' named entity 'centrifugation' named entity 'PerkinElmer' named entity 'Japan' named entity 'vector' named entity 'lysine' named entity 'kDa' named entity 'wavelength' named entity 'CAT assay' named entity '3.5' named entity 'S30' named entity 'OD600' named entity 'S30' named entity 'proline' named entity 'centrifugation' named entity 'centrifuged' named entity 'NaCl' named entity 'stop codons' named entity 'kanamycin' named entity 'polyethylenimine' named entity 'DNA polymerase' named entity 'PCR' named entity 'glutathione S-transferase' named entity 'batch mode' named entity 'plasmid' named entity 'cell-free' named entity 'amino acid' named entity 'absorbance' named entity 'Gam protein' named entity 'imidazole' named entity 'Stansted' named entity 'lysis buffer' named entity 'vector' named entity 'TFF' named entity 'PCR' named entity 'IPTG' named entity 'PCR' named entity 'pH 8' named entity 'supernatant' named entity 'rhamnose' named entity 'centrifuged' named entity 'centrifuged' named entity 'SYBR Green' named entity 'centrifugation' named entity 'GE Healthcare' named entity 'nuclease' named entity 'TFF' named entity 'IPTG' named entity 'liquid nitrogen' named entity 'A260' named entity 'GST' named entity 'log phase' named entity 'tangential flow filtration' named entity 'GE Healthcare' named entity 'Japan' named entity 'UniProt' named entity 'lysis buffer' named entity 'cell-free protein synthesis' named entity 'S30' named entity 'A260' named entity 'A260' named entity 'ammonium sulfate' named entity 'protein synthesis' named entity '7,500' named entity 'Merck Millipore' named entity '7.5' named entity 'GE Healthcare' named entity 'diafiltration' named entity 'S30' named entity 'OD600' named entity 'retention time' named entity 'plasmid' named entity 'S30' named entity 'S30' named entity 'oligonucleotides' named entity 'S30' named entity 'cell-free'

Attributes

Values

Creator

Shirouzu, Mikako
Takemoto, Chie
Hanada, Kazuharu
Katsura, Kazushige
Matsuda, Takayoshi
Ohsawa, Noboru
Sakamoto, Kensaku
Tomabechi, Yuri
Yonemochi, Mayumi

is schema:about of

named entity 'cognate'
named entity 'phosphoenolpyruvate'
named entity 'ampicillin'
named entity 'culture medium'
named entity 'ultrafiltration'
named entity 'NaCl'
named entity 'S30'
named entity 'alkaline'
named entity 'NaCl'
named entity 'recombinant'
named entity 'alkaline'
named entity 'cell-free'
named entity 'T7 RNA polymerase'
named entity 'NaCl'
named entity 'GST'
named entity 'centrifugation'
named entity 'PerkinElmer'
named entity 'Japan'
named entity 'vector'
named entity 'lysine'
named entity 'kDa'
named entity 'wavelength'
named entity 'CAT assay'
named entity '3.5'
named entity 'S30'
named entity 'OD600'
named entity 'S30'
named entity 'proline'
named entity 'centrifugation'
named entity 'centrifuged'
named entity 'NaCl'
named entity 'stop codons'
named entity 'kanamycin'
named entity 'polyethylenimine'
named entity 'DNA polymerase'
named entity 'PCR'
named entity 'glutathione S-transferase'
named entity 'batch mode'
named entity 'plasmid'
named entity 'cell-free'
named entity 'amino acid'
named entity 'absorbance'
named entity 'Gam protein'
named entity 'imidazole'
named entity 'Stansted'
named entity 'lysis buffer'
named entity 'vector'
named entity 'TFF'
named entity 'PCR'
named entity 'IPTG'
named entity 'PCR'
named entity 'pH 8'
named entity 'supernatant'
named entity 'rhamnose'
named entity 'centrifuged'
named entity 'centrifuged'
named entity 'SYBR Green'
named entity 'centrifugation'
named entity 'GE Healthcare'
named entity 'nuclease'
named entity 'TFF'
named entity 'IPTG'
named entity 'liquid nitrogen'
named entity 'A260'
named entity 'GST'
named entity 'log phase'
named entity 'tangential flow filtration'
named entity 'GE Healthcare'
named entity 'Japan'
named entity 'UniProt'
named entity 'lysis buffer'
named entity 'cell-free protein synthesis'
named entity 'S30'
named entity 'A260'
named entity 'A260'
named entity 'ammonium sulfate'
named entity 'protein synthesis'
named entity '7,500'
named entity 'Merck Millipore'
named entity '7.5'
named entity 'GE Healthcare'
named entity 'diafiltration'
named entity 'S30'
named entity 'OD600'
named entity 'retention time'
named entity 'plasmid'
named entity 'S30'
named entity 'S30'
named entity 'oligonucleotides'
named entity 'S30'
named entity 'cell-free'

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