About: Application of Epstein–Barr Virus for Optimization of Immortalized B-lymphocyte Production as a Positive Control in Genetic Studies

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Application of Epstein–Barr Virus for Optimization of Immortalized B-lymphocyte Production as a Positive Control in Genetic Studies
Creator	Ghanbarian, Maryam Mirian, Mina Moghim, Sharareh Salehi, Mansoor Tousizadeh, Behnaz Tousizadeh, Sepideh Zaboli, Fatemeh Chaleshtori, Ahmad
Source	PMC
abstract	BACKGROUND: Infection of B-cells with Epstein–Barr virus (EBV) leads to more and subsequent immortalization. This is considered as the method of choice for generating lymphoblastoid cell lines (LCLs). Producing LCLs, although very useful but is very time consuming and troublesome, drives the requirement for quicker and more reliable methods for EBV-driven B-cell transformation. MATERIALS AND METHODS: After successfully production of LCLs, different parameters including temperature, serum concentration, type of culture medium, and CO(2) concentration were evaluated on EBV-transformed B-cells. In this study, we were able to produce LCLs and optimize condition. RESULTS: The best condition for generating LCLs was 37°C, 5% CO(2), 20% fasting blood sugar, and RPMI 1640. The study results were to establish a reliable method for producing LCLs that can be used to produce immortalized B-cells from almost any sources. CONCLUSION: This can help with tumorgenecity studies, as well as producing control material for rare genetic disorders and so on. The aim of this study was to determine optimized condition for reliable and reproducible LCLs from different sources.
has issue date	2017-07-14(xsd:dateTime)
bibo:doi	10.4103/2277-9175.210659
bibo:pmid	28808646
has license	cc-by-nc-sa
schema:url	https://doi.org/10.4103/2277-9175.210659
resource representing a document's title	Application of Epstein–Barr Virus for Optimization of Immortalized B-lymphocyte Production as a Positive Control in Genetic Studies
has PubMed Central identifier	PMC5539668
has PubMed identifier	28808646
schema:publication	Adv Biomed Res
resource representing a document's body	covid:PMC5539668#body_text
is schema:about of	named entity 'B-lymphocyte' named entity 'Epstein-Barr Virus' named entity 'gel electrophoresis' named entity 'FBS' named entity 'Ficoll' named entity 'EBV' named entity 'lymphoblastoid cells' named entity 'Sir Michael Anthony Epstein' named entity 'lymphocytes' named entity 'culture medium' named entity 'infection' named entity 'blood sampling' named entity 'FBS' named entity 'genetic disorders' named entity 'RPMI' named entity 'primers' named entity 'viruses' named entity 'fasting blood sugar' named entity 'internal control' named entity 'EBV' named entity 'CO2' named entity 'PBS' named entity 'B-lymphocytes' named entity 'CO2' named entity 'lymphocyte' named entity '37°C' named entity 'Hypaque' named entity 'cell line' named entity 'RPMI' named entity 'virus' named entity 'cell line' named entity 'PBS' named entity 'agarose' named entity 'terminal differentiation' named entity 'liquid nitrogen' named entity 'genetic analysis' named entity 'genetic epidemiology' named entity 'morphology' named entity 'virology' named entity 'clusters of cells' named entity 'RPMI' named entity 'Histocompatibility' named entity '1.6' named entity 'culture medium' named entity 'phosphate-buffered saline' named entity 'HLA' named entity 'RPMI' named entity 'FCS' named entity 'CO2' named entity 'CO2' named entity 'cyclosporin' named entity 'PCR' named entity '36.5°C' named entity 'PCR amplification' named entity 'primers' named entity 'cell cycle arrest' named entity 'EBV' named entity 'FCS' named entity 'mononucleosis' named entity 'buffy coat' named entity 'cell line' named entity 'B-lymphocytes' named entity 'lymphocyte' named entity 'serological' named entity 'PCR amplification' named entity 'supernatant' named entity 'CO2' named entity 'DMEM' named entity 'polymerase chain reaction' named entity 'microscope' named entity 'FBS' named entity 'fibroblastic' named entity 'virus' named entity 'internal control' named entity 'PCR'

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