About: Reprogramming of sugar transport pathways in Escherichia coli using a permeabilized SecY protein‐translocation channel

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About: Reprogramming of sugar transport pathways in Escherichia coli using a permeabilized SecY protein‐translocation channel Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Reprogramming of sugar transport pathways in Escherichia coli using a permeabilized SecY protein‐translocation channel
Creator	Guo, Qiang Fan, Li-Hai Jiang, Yang Mei, Sen Xie, Chong Hao, \| Tan, Tian-Wei Zhang, Shi-Ding
Source	Medline; PMC
abstract	In the initial step of sugar metabolism, sugar‐specific transporters play a decisive role in the passage of sugars through plasma membranes into cytoplasm. The SecY complex (SecYEG) in bacteria forms a membrane channel responsible for protein translocation. The present work shows that permeabilized SecY channels can be used as nonspecific sugar transporters in Escherichia coli. SecY with the plug domain deleted allowed the passage of glucose, fructose, mannose, xylose, and arabinose, and, with additional pore‐ring mutations, facilitated lactose transport, indicating that sugar passage via permeabilized SecY was independent of sugar stereospecificity. The engineered E. coli showed rapid growth on a wide spectrum of monosaccharides and benefited from the elimination of transport saturation, improvement in sugar tolerance, reduction in competitive inhibition, and prevention of carbon catabolite repression, which are usually encountered with native sugar uptake systems. The SecY channel is widespread in prokaryotes, so other bacteria may also be engineered to utilize this system for sugar uptake. The SecY channel thus provides a unique sugar passageway for future development of robust cell factories for biotechnological applications.
has issue date	2020-02-20(xsd:dateTime)
bibo:doi	10.1002/bit.27306
bibo:pmid	32048725
has license	no-cc
sha1sum (hex)	9eca0443ef870b212ee1d78d59aff77c227ba1ca
schema:url	https://doi.org/10.1002/bit.27306
resource representing a document's title	Reprogramming of sugar transport pathways in Escherichia coli using a permeabilized SecY protein‐translocation channel
has PubMed Central identifier	PMC7147117
has PubMed identifier	32048725
schema:publication	Biotechnol Bioeng
resource representing a document's body	covid:9eca0443ef870b212ee1d78d59aff77c227ba1ca#body_text
is schema:about of	named entity 'ESCHERICHIA COLI' named entity 'STEP' named entity 'SUGAR METABOLISM' named entity 'MEMBRANE CHANNEL' named entity 'sugar metabolism' named entity 'specific transporters' named entity 'membrane channel' named entity 'glucose' named entity 'protein' named entity 'sugar' named entity 'Escherichia coli' named entity 'FLP recognition target' named entity 'SecY' named entity 'glucose' named entity 'glucose transport' named entity 'sugar' named entity 'assay' named entity 'E. coli' named entity 'outer membrane' named entity 'carbon' named entity 'kmol' named entity 'fermentation' named entity 'SecY' named entity 'FLP recombinase' named entity 'oxygen' named entity 'competitive inhibition' named entity 'plug domain' named entity 'passive transport' named entity 'wild-type' named entity 'glucose' named entity 'mutation' named entity 'specific transporters' named entity 'sugar' named entity 'diffusion' named entity 'E. coli' named entity 'envelope protein' named entity 'gene' named entity 'permeation' named entity 'green fluorescent protein' named entity 'cytoplasm' named entity 'glucose' named entity 'transmembrane' named entity 'ampicillin' named entity 'CCR' named entity 'amino acid residues' named entity 'plasma membrane' named entity 'E. coli' named entity 'arabinose' named entity 'molecular binding' named entity 'conserved protein' named entity 'carbohydrate' named entity '37°C' named entity 'kanamycin' named entity 'Active transport' named entity 'sugar' named entity 'LacY' named entity 'specific transporters' named entity 'primers' named entity 'SecY' named entity 'SecY' named entity 'sugar' named entity 'polypeptides' named entity 'primers' named entity 'polypeptide' named entity '37°C' named entity 'glucose' named entity 'arabinose' named entity 'glucose' named entity 'sugar' named entity 'carbon catabolite repression' named entity 'sugar' named entity 'lactose' named entity 'gene' named entity 'arabinose'

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