About: A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus

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About: A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus
Creator	Lu, Z Osorio, Fernando Pattnaik, Asit Galeota, Judith Kutish, Gerald Truong, Ha
source	Elsevier; Medline; PMC
abstract	Abstract The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that of the parental virus. Primer extension analysis revealed that during replication, the viral polymerase corrected the two nonviral guanosine residues present at the 5′ terminus of the transfected transcripts. Animal studies showed that the cloned virus induced hyperthermia, persistent viremia, and antibody response, similar to that observed with the parental virus. Contact transmission occurred rapidly within 3 days of introduction of naı̈ve pigs into the group of clone virus-inoculated pigs. These results suggest that the cloned virus retains the in vivo virulence and contagion properties of the parental virus, thus, providing the background for reverse genetics manipulation in systematic examination of attenuation and virulence phenotypes.
has issue date	2004-08-01(xsd:dateTime)
bibo:doi	10.1016/j.virol.2004.04.046
bibo:pmid	15246270
has license	els-covid
sha1sum (hex)	9c7828431aec5b114f83e587b61b4d77d4b641f5
schema:url	https://doi.org/10.1016/j.virol.2004.04.046
resource representing a document's title	A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus
has PubMed Central identifier	PMC7127741
has PubMed identifier	15246270
schema:publication	Virology
resource representing a document's body	covid:9c7828431aec5b114f83e587b61b4d77d4b641f5#body_text
is schema:about of	named entity 'porcine' named entity 'Animal studies' named entity 'cDNA' named entity 'virulence' named entity 'hyperthermia' named entity 'clone' named entity 'virulence' named entity 'parental' named entity 'PATHOGENIC' named entity 'CLONE' named entity 'GROWTH CHARACTERISTICS' named entity 'ANTIBODY RESPONSE' named entity 'GENETICS MANIPULATION' named entity 'PERSISTENT VIREMIA' named entity 'SUGGEST' named entity 'OCCURRED' named entity 'CDNA CLONE' named entity 'BACKGROUND' named entity 'CONTACT TRANSMISSION' named entity 'GROUP' named entity 'VIRULENCE' named entity 'GENERATED' named entity 'VIRUS' named entity 'PARENTAL' named entity 'VIRULENCE' named entity 'RETAINS' named entity 'TRANSCRIPTS' named entity 'CELLS' named entity 'VIRAL GENOME' named entity 'TERMINUS' named entity 'CORRECTED' named entity 'IN VITRO' named entity 'OBSERVED' named entity 'LENGTH' named entity 'PROVIDING' named entity 'PIGS' named entity 'INFECTIOUS' named entity 'DAYS' named entity 'VIRUS' named entity 'IN VIVO' named entity 'CDNA CLONE' named entity 'INFECTIOUS' named entity 'GUANOSINE' named entity 'CAPPED' named entity 'RESULTS' named entity 'REPLICATION' named entity 'RAPIDLY' named entity 'PARENTAL' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'RETAINS' named entity 'SIMILAR' named entity 'TRANSFECTION' named entity 'REVERSE GENETICS' named entity 'DERIVED' named entity 'PROPERTIES' named entity 'HIGHLY' named entity 'INDUCED HYPERTHERMIA' named entity 'CLONED' named entity 'REVEALED' named entity 'MARC' named entity 'HIGHLY' named entity 'DETERMINED' named entity 'NUCLEOTIDE SEQUENCE' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'PHENOTYPES' named entity 'PATHOGENIC' named entity 'EXAMINATION' named entity 'ANIMAL STUDIES' named entity '145' named entity 'ATTENUATION' named entity 'PRIMER EXTENSION ANALYSIS' named entity 'IN VIVO' named entity 'INTRODUCTION' named entity 'PROPERTIES' named entity 'PRESENT' named entity 'THESE'

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