About: Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture

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About: Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture
Creator	Beugeling, Corrine Zevenhoven-Dobbe, Jessika Gallay, Philippe Chatterji, Udayan Szuhai, Karoly Snijder, Eric De Wilde, Adriaan Posthuma, Clara De Jong, Danielle
Source	Elsevier; Medline; PMC
abstract	Abstract Cyclophilin A (CypA) is an important host factor in the replication of a variety of RNA viruses. Also the replication of several nidoviruses was reported to depend on CypA, although possibly not to the same extent. These prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. Here, we investigated the CypA dependence of three distantly related nidoviruses that can all replicate in Huh7 cells: the arterivirus equine arteritis virus (EAV), the alphacoronavirus human coronavirus 229E (HCoV-229E), and the betacoronavirus Middle East respiratory syndrome coronavirus (MERS-CoV). The replication of these viruses was compared in the same parental Huh7 cells and in CypA-knockout Huh7 cells generated using CRISPR/Cas9-technology. CypA depletion reduced EAV yields by ~ 3-log, whereas MERS-CoV progeny titers were modestly reduced (3-fold) and HCoV-229E replication was unchanged. This study reveals that the replication of nidoviruses can differ strikingly in its dependence on cellular CypA.
has issue date	2018-04-30(xsd:dateTime)
bibo:doi	10.1016/j.virol.2017.11.022
bibo:pmid	29249267
has license	els-covid
sha1sum (hex)	99525a04b200a0b567c1981222bc1a643d7bf2fd
schema:url	https://doi.org/10.1016/j.virol.2017.11.022
resource representing a document's title	Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture
has PubMed Central identifier	PMC7112125
has PubMed identifier	29249267
schema:publication	Virology
resource representing a document's body	covid:99525a04b200a0b567c1981222bc1a643d7bf2fd#body_text
is schema:about of	named entity 'striking' named entity 'cell culture' named entity 'CORONAVIRUSES' named entity 'REPLICATION' named entity 'DEPENDENCE' named entity 'CYCLOPHILIN A' named entity 'ARTERIVIRUSES' named entity 'CELL CULTURE' named entity 'THEIR' named entity 'STRIKING' named entity 'DISPLAY' named entity 'cell culture' named entity 'cyclophilin' named entity 'nested set' named entity 'HCoV-NL63' named entity 'chromosome' named entity 'MERS-CoV' named entity 'image analysis' named entity 'short arm' named entity 'protein trafficking' named entity 'arterivirus' named entity 'coronaviruses' named entity 'nidovirus' named entity 'shRNA' named entity 'clustered regularly interspaced short palindromic repeat' named entity 'Western blot analysis' named entity 'Huh7' named entity 'PPIB' named entity 'Nidovirus' named entity 'Huh7' named entity 'amino acids' named entity 'plasmid' named entity 'log reduction' named entity 'PCR' named entity 'PPIA' named entity 'hepatitis C virus' named entity 'nidovirus' named entity 'cell clones' named entity 'gene' named entity 'FCoV' named entity 'cDNA' named entity 'PPIA' named entity 'HCoV-229E' named entity 'lysate' named entity 'gene' named entity 'Huh7' named entity 'mRNA' named entity '293T' named entity 'cell lines' named entity 'nuclease' named entity 'CypC' named entity 'cell clones' named entity 'chaperones' named entity 'gRNA' named entity 'HIV-1' named entity 'HCV' named entity 'Bio-Rad' named entity 'Virology' named entity 'PPIA' named entity 'De Wilde' named entity 'gene' named entity '37°C' named entity 'Huh7' named entity 'alphacoronavirus' named entity 'isochromosome' named entity 'transfection' named entity 'Huh7' named entity 'Cas9' named entity 'cell lines' named entity 'alphacoronaviruses' named entity 'polyprotein' named entity 'PPIA' named entity 'virus replication'

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