About: Human SCARB2-Mediated Entry and Endocytosis of EV71

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Human SCARB2-Mediated Entry and Endocytosis of EV71 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Human SCARB2-Mediated Entry and Endocytosis of EV71
Creator	Chitra, E Hsiao, K-N Lin, H-Y Lin, Y-W Tsou, Y-L
Source	Medline; PMC
abstract	Enterovirus (EV) 71 infection is known to cause hand-foot-and-mouth disease (HFMD) and in severe cases, induces neurological disorders culminating in fatality. An outbreak of EV71 in South East Asia in 1997 affected over 120,000 people and caused neurological disorders in a few individuals. The control of EV71 infection through public health interventions remains minimal and treatments are only symptomatic. Recently, human scavenger receptor class B, member 2 (SCARB2) has been reported to be a cellular receptor of EV71. We expressed human SCARB2 gene in NIH3T3 cells (3T3-SCARB2) to study the mechanisms of EV71 entry and infection. We demonstrated that human SCARB2 serves as a cellular receptor for EV71 entry. Disruption of expression of SCARB2 using siRNAs can interfere EV71 infection and subsequent inhibit the expression of viral capsid proteins in RD and 3T3-SCARB2 but not Vero cells. SiRNAs specific to clathrin or dynamin or chemical inhibitor of clathrin-mediated endocytosis were all capable of interfering with the entry of EV71 into 3T3-SCARB2 cells. On the other hand, caveolin specific siRNA or inhibitors of caveolae-mediated endocytosis had no effect, confirming that only clathrin-mediated pathway was involved in EV71 infection. Endocytosis of EV71 was also found to be pH-dependent requiring endosomal acidification and also required intact membrane cholesterol. In summary, the mechanism of EV71 entry through SCARB2 as the receptor for attachment, and its cellular entry is through a clathrin-mediated and pH-dependent endocytic pathway. This study on the receptor and endocytic mechanisms of EV71 infection is useful for the development of effective medications and prophylactic treatment against the enterovirus.
has issue date	2012-01-17(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0030507
bibo:pmid	22272359
has license	cc-by
sha1sum (hex)	98e52cb0b81f381ecd6138f73e6596de35d7566f
schema:url	https://doi.org/10.1371/journal.pone.0030507
resource representing a document's title	Human SCARB2-Mediated Entry and Endocytosis of EV71
has PubMed Central identifier	PMC3260287
has PubMed identifier	22272359
schema:publication	PLoS One
resource representing a document's body	covid:98e52cb0b81f381ecd6138f73e6596de35d7566f#body_text
is schema:about of	named entity 'attachment' named entity 'SCARB2' named entity 'caused' named entity 'specific' named entity 'human' named entity 'SCARB2' named entity 'The' named entity 'capsid proteins' covid:arg/98e52cb0b81f381ecd6138f73e6596de35d7566f named entity 'severe' named entity 'control' named entity 'siRNAs' named entity 'specific' named entity 'public health' named entity 'cases' named entity 'subsequent' named entity 'minimal' named entity 'SiRNAs' named entity 'receptor' named entity 'chemical' named entity 'dynamin' named entity 'SCARB2' named entity 'SCARB2' named entity 'HFMD' named entity 'EV71' named entity 'EV71' named entity 'enterovirus' named entity 'infection' named entity 'EV71' named entity 'infection' named entity 'infection' named entity 'EV71' named entity 'clathrin' named entity 'EV71' named entity 'endosomal' named entity 'cellular receptor' named entity 'South East Asia' named entity 'neurological disorders' named entity 'Endocytosis' named entity 'study' named entity 'transfection' named entity 'caveolae' named entity 'human lung' named entity 'host cells' named entity 'E59' named entity 'PMSF' named entity 'supernatant' named entity 'Western blotting' named entity 'sodium deoxycholate' named entity 'endosomes' named entity 'SCARB2' named entity 'EV71' named entity 'cyclodextrin' named entity 'African green monkey' named entity 'PSGL-1' named entity 'EV71' named entity 'Invitrogen' named entity 'Osaka' named entity 'infection' named entity 'SCARB2' named entity 'plasma membrane' named entity 'peroxidase' named entity 'ebola virus' named entity 'SCARB2' named entity 'phagocytosis' named entity 'FBS' named entity 'Taiwan' named entity 'FBS' named entity '5.5' named entity 'clathrin-mediated endocytosis' named entity 'antibody' named entity 'Vero cells' named entity 'ATCC' named entity 'clathrin-mediated endocytosis' named entity 'Vero cells' named entity 'viruses' named entity 'western blotting'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software