About: Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection
Creator	Benitez, Alvaro Crabb, Donna Dluhy, Richard Henderson, Kelley Krause, Duncan Ratliff, Amy Sheppard, Edward Waites, Ken Winchell, Jonas Atkinson, T
Source	Medline; PMC
abstract	Mycoplasma pneumoniae is a cell wall-less bacterial pathogen of the human respiratory tract that accounts for > 20% of all community-acquired pneumonia (CAP). At present the most effective means for detection and strain-typing is quantitative polymerase chain reaction (qPCR), which can exhibit excellent sensitivity and specificity but requires separate tests for detection and genotyping, lacks standardization between available tests and between labs, and has limited practicality for widespread, point-of-care use. We have developed and previously described a silver nanorod array-surface enhanced Raman Spectroscopy (NA-SERS) biosensing platform capable of detecting M. pneumoniae with statistically significant specificity and sensitivity in simulated and true clinical throat swab samples, and the ability to distinguish between reference strains of the two main genotypes of M. pneumoniae. Furthermore, we have established a qualitative lower endpoint of detection for NA-SERS of < 1 genome equivalent (cell/μl) and a quantitative multivariate detection limit of 5.3 ± 1 cells/μl. Here we demonstrate using partial least squares- discriminatory analysis (PLS-DA) of sample spectra that NA-SERS correctly identified M. pneumoniae clinical isolates from globally diverse origins and distinguished these from a panel of 12 other human commensal and pathogenic mycoplasma species with 100% cross-validated statistical accuracy. Furthermore, PLS-DA correctly classified by strain type all 30 clinical isolates with 96% cross-validated accuracy for type 1 strains, 98% cross-validated accuracy for type 2 strains, and 90% cross-validated accuracy for type 2V strains.
has issue date	2015-06-29(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0131831
bibo:pmid	26121242
has license	cc0
sha1sum (hex)	974446ae540829dc1b4c7ce8ea1b8dd3843fb3dc
schema:url	https://doi.org/10.1371/journal.pone.0131831
resource representing a document's title	Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection
has PubMed Central identifier	PMC4487258
has PubMed identifier	26121242
schema:publication	PLoS One
resource representing a document's body	covid:974446ae540829dc1b4c7ce8ea1b8dd3843fb3dc#body_text
is schema:about of	named entity 'TYPING' named entity 'DETECTION' named entity 'tests' named entity 'genotypes' named entity 'detecting' named entity 'spectra' named entity 'commensal' named entity 'detection limit' named entity 'panel' named entity 'sensitivity' named entity 'Nanorod' named entity 'Mycoplasma pneumoniae' named entity 'CLINICAL' named entity 'USING' named entity 'TYPE 2' named entity 'TYPING' named entity 'COMMUNITY-ACQUIRED PNEUMONIA' named entity 'BUT' named entity 'DESCRIBED' named entity 'PANEL' named entity 'ESTABLISHED' named entity 'VALIDATED' named entity 'COMMENSAL' named entity 'STRAIN' named entity 'PLATFORM' named entity 'TYPE 1' named entity '100%' named entity 'SURFACE ENHANCED RAMAN SPECTROSCOPY' named entity 'SENSITIVITY' named entity 'DIVERSE' named entity 'STRAINS' named entity 'LOWER' named entity 'PNEUMONIAE' named entity 'EXCELLENT' named entity 'SPECIFICITY' named entity '20%' named entity 'CAPABLE' named entity 'PREVIOUSLY' named entity 'TYPE' named entity 'WIDESPREAD' named entity 'PLS-DA' named entity 'SPECIES' named entity 'ANALYSIS' named entity 'THROAT SWAB' named entity 'BIOSENSING' named entity 'CELLS' named entity 'EFFECTIVE' named entity 'HUMAN' named entity 'EQUIVALENT' named entity 'LACKS' named entity 'CLASSIFIED' named entity 'PRESENT' named entity 'EXHIBIT' named entity 'MYCOPLASMA' named entity 'QUALITATIVE' named entity 'STATISTICALLY SIGNIFICANT' named entity 'CROSS' named entity 'STANDARDIZATION' named entity 'DETECTING' named entity 'PATHOGENIC' named entity 'AVAILABLE' named entity 'MYCOPLASMA PNEUMONIAE' named entity 'DETECTION' named entity 'SEPARATE' named entity 'PARTIAL LEAST SQUARES' named entity 'QUANTITATIVE POLYMERASE CHAIN REACTION' named entity 'THESE' named entity 'SIMULATED' named entity 'STRAIN TYPE' named entity 'TESTS' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'A CELL'

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