About: The Intraleader AUG Nucleotide Sequence Context is Important for Equine Arteritis Virus Replication

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The Intraleader AUG Nucleotide Sequence Context is Important for Equine Arteritis Virus Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The Intraleader AUG Nucleotide Sequence Context is Important for Equine Arteritis Virus Replication
Creator	Archambault, Denis Archambault, D Ali, A De Vries, A Peter, A De Vries, Antoine Ae, Kheyar Kheyar, Ae Rottier, Ae Rottier, J
source	Medline; PMC
abstract	The 5(-terminal leader sequence of the equine arteritis virus (EAV) genome contains an open reading frame (ORF) with an AUG codon in a suboptimal context for initiation of protein synthesis. To investigate the significance of this intraleader ORF (ILO), an expression plasmid was generated carrying a DNA copy of the subgenomic mRNA7 behind a T7 promoter. Capped RNA transcribed from this construct was shown to direct, in an in vitro translation system, the synthesis of leader peptide as well as N protein. Site-directed mutations aimed to either optimize or weaken the sequence context of the ILO start codon affected leader peptide synthesis as predicted; no peptide was detected when the initiation codon was incapacitated. Translation of the downstream N gene was inversely affected by leader peptide production, consistent with a ribosomal leaky scanning mechanism. To investigate the role of the leader peptide in the EAV replication life cycle we generated, using an infectious EAV cDNA clone, two mutant viruses in one of which the ILO start codon was in an optimal Kozak context for translation initiation while in the other the codon was again incapacitated. Surprizingly, both mutant viruses were equally viable and exhibited similar phenotypes in BHK-21 cells. However, their replication kinetics and viral yields were reduced relative to that of the wild-type parental virus, as were their plaque sizes. Importantly, the mutations introduced into the viruses appeared to be rapidly and precisely repaired upon passaging. Already after one viral passage a significant fraction of the viruses had regained the wild-type sequence as well as its phenotype. The results demonstrate that EAV replication is not dependent on the synthesis of the intraleader peptide. Rather, the leader peptide does not seem to have any function in the EAV life cycle. As we discuss, the available data indicate that the ILO 5( nucleotide sequence per se, not its functioning in translation initiation, is of critical importance for EAV replication.
has issue date	2006-01-01(xsd:dateTime)
bibo:doi	10.1007/s11262-005-0030-z
bibo:pmid	16791420
has license	no-cc
sha1sum (hex)	971e69fa9c53f33f8bf6c49dae6f0cda10ed6776
schema:url	https://doi.org/10.1007/s11262-005-0030-z
resource representing a document's title	The Intraleader AUG Nucleotide Sequence Context is Important for Equine Arteritis Virus Replication
has PubMed Central identifier	PMC7088518
has PubMed identifier	16791420
schema:publication	Virus Genes
resource representing a document's body	covid:971e69fa9c53f33f8bf6c49dae6f0cda10ed6776#body_text
is schema:about of	named entity 'kinetics' named entity 'peptide synthesis' named entity 'peptide' named entity 'fraction' named entity 'leader' named entity 'viruses' named entity 'generated' named entity 'EAV' named entity 'plasmid' named entity 'CONTEXT' named entity 'GENOME' named entity 'CDNA CLONE' named entity 'INTRODUCED INTO' named entity 'WILD-TYPE' named entity 'INFECTIOUS' named entity 'COPY' named entity 'TRANSCRIBED' named entity 'OPEN READING FRAME' named entity 'ONE OF' covid:arg/971e69fa9c53f33f8bf6c49dae6f0cda10ed6776 named entity 'EAV' named entity 'repaired' named entity 'dependent' named entity 'EAV' named entity 'ILO' named entity 'mutations' named entity 'equally' named entity 'optimal' named entity 'leaky scanning' named entity 'leader' named entity 'gene' named entity 'nucleotide sequence' named entity 'mutant' named entity 'initiation' named entity 'yields' named entity 'consistent' named entity 'generated' named entity 'equine arteritis virus' named entity 'life cycle' named entity 'life cycle' named entity 'RNA' named entity 'T7 promoter' named entity 'leader peptide' named entity 'phenotype' named entity 'Kozak' named entity 'initiation codon' named entity 'peptide' named entity 'EAV' named entity 'ILO' named entity 'leaky scanning' named entity 'start codon' named entity 'equine arteritis virus' named entity 'nucleotide sequence' named entity 'equine arteritis virus' named entity 'direct' named entity 'leader sequence' named entity 'antisense' named entity 'fetal bovine serum' named entity 'viral titer' named entity 'EAV' named entity 'avian myeloblastosis virus' named entity 'EcoRV' named entity 'atg' named entity 'transfection' named entity 'protein' named entity 'ORFs' named entity 'atg' named entity 'antiserum' named entity 'ORF' named entity 'supernatants' named entity 'SDS' named entity 'formaldehyde'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software