About: The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples

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About: The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples
Creator	Bennett, Susan Gunson, Rory
Source	Elsevier; Medline; PMC
abstract	Abstract Viral gastroenteritis is a major health problem with significant morbidity and economic consequences. Viral gastroenteritis is caused by a number of viruses, including norovirus, rotavirus, adenovirus, astrovirus, and sapovirus. Conventional diagnosis is based on direct antigen detection and electron microscopy, however enzyme immunoassay’s are insensitive and not available for all relevant pathogens, and electron microscope (EM) is no longer routinely carried out in most laboratories. Most laboratories now offer norovirus real-time PCR testing however the availability of other assays is variable. Commercial methods for the detection of inflectional intestinal disease (IID) are available but these can be expensive and are not commonly used. This paper describes the development of a single multiplex assay for the simultaneous detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples. The multiplex was evaluated by assessing endpoint sensitivity, specificity, panel of clinical samples, quality control (QC) panel and the robustness and reproducibility of the multiplex.
has issue date	2017-04-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2016.12.016
bibo:pmid	28040514
has license	els-covid
sha1sum (hex)	953fe24528a157cdb72e21758bc15ca0b2fbee4f
schema:url	https://doi.org/10.1016/j.jviromet.2016.12.016
resource representing a document's title	The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples
has PubMed Central identifier	PMC7173313
has PubMed identifier	28040514
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:953fe24528a157cdb72e21758bc15ca0b2fbee4f#body_text
is schema:about of	named entity 'specificity' named entity 'Commercial' named entity 'panel' named entity 'direct' named entity 'disease' named entity 'astrovirus' named entity 'USED' named entity 'PAPER' named entity 'VIRUSES' named entity 'SENSITIVITY' named entity 'STOOL' named entity 'SIGNIFICANT' named entity 'DIAGNOSIS' named entity 'A MAJOR' named entity 'sapovirus' named entity 'problem' named entity 'samples' named entity 'astrovirus' named entity 'Viral' named entity 'based' named entity 'number' named entity 'gastroenteritis' named entity 'rotavirus' named entity 'detection' named entity 'adenovirus' named entity 'variable' named entity 'simultaneous' named entity 'adenovirus' named entity 'reproducibility' named entity 'sapovirus' named entity 'rotavirus' named entity 'rotavirus' named entity 'sapovirus' named entity 'viruses' named entity 'astrovirus' named entity 'enzyme immunoassay' named entity 'sapovirus' named entity 'viruses' named entity 'gastroenteritis' named entity 'PCR' named entity 'supernatant' named entity 'viruses' named entity 'West of Scotland' named entity 'adenovirus' named entity 'false negative results' named entity 'rotavirus' named entity 'rotavirus' named entity 'serotypes' named entity 'norovirus' named entity 'Adenovirus' named entity 'gastroenteritis' named entity 'rotavirus' named entity 'real-time PCR' named entity 'sensitivity and specificity' named entity 'multiplex assay' named entity 'sensitive and specific' named entity 'rotavirus' named entity 'rotavirus' named entity 'eluted' named entity 'real-time PCR' named entity 'adenovirus' named entity 'nucleic acid' named entity 'sapovirus' named entity 'PCR' named entity 'health problem' named entity 'RNA extraction' named entity 'primers' named entity 'Real-time PCR' named entity 'gastroenteritis' named entity 'adenovirus' named entity 'sensitive and specific' named entity 'adenovirus' named entity 'infection' named entity 'rotavirus' named entity 'C V' named entity 'detection limit' named entity 'sapovirus' named entity 'Sapovirus' named entity 'norovirus' named entity 'cell culture'

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