About: Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens

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About: Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens
Creator	Zhang, Yong Li, Jin Zhang, Chen Ma, Xuejun Shen, Hongwei Shi, Weixian Wang, Ji Li, Wang Guan, Li Mo, Haolian Wang, Shulian Zeng, Huashu Zhu, Bingqing Amaral, Lara Brazil, Cruz-Fiocruz, Oswaldo Fortini, Rafaella Malgorzata, Anna Mikaszewska-Sokolewicz, Queiroz, Grenfell
Source	Medline; PMC
abstract	A large number of viral and bacterial organisms are responsible for community-acquired pneumonia (CAP) which contributes to substantial burden on health management. A new resequencing microarray (RPM-IVDC1) associated with targeted multiplex PCR was recently developed and validated for multiple respiratory viruses detection and discrimination. In this study, we evaluated the capability of RPM-IVDC1 for simultaneous identification of multiple viral and bacterial organisms. The nasopharyngeal aspirates (NPAs) of 110 consecutive CAP patients, aged from 1 month to 96 years old, were collected from five distinct general hospitals in Beijing during 1-year period. The samples were subjected to the RPM-IVDC1 established protocol as compared to a real-time PCR (qRT-PCR), which was used as standard. The results of virus detection were consistent with those previously described. A total of 37 of Streptococcus pneumoniae, 14 of Haemophilus influenzae, 10 of Mycoplasma pneumoniae, two of Klebsiella pneumoniae and one of Moraxella catarrhalis were detected by RPM-IVDC1. The sensitivities and specificities were compared with those of qRT-PCR for S. pneumoniae (100, 100%, respectively), H. influenzae (92.3, 97.9%, respectively), M. pneumoniae (69.2, 99.0%, respectively), K. pneumoniae (100, 100%, respectively), and M. catarrhalis (100, 100%, respectively). Additional 22 of Streptococcus spp., 24 of Haemophilus spp. and 16 of Neisseria spp. were identified. In addition, methicillin-resistant and carbapenemases allele were also found in nine of Staphylococcus spp. and one of K. pneumoniae, respectively. These results demonstrated the capability of RPM-IVDC1 for simultaneous detection of broad-spectrum respiratory pathogens in complex backgrounds and the advantage of accessing to the actual sequences, showing great potential use of epidemic outbreak investigation. The detection results should be carefully interpreted when introducing this technique in the clinical diagnostics.
has issue date	2015-05-28(xsd:dateTime)
bibo:doi	10.3389/fmicb.2015.00532
bibo:pmid	26074910
has license	cc-by
sha1sum (hex)	9223fd6478822761845291fe2ac429ca6ddbb45a
schema:url	https://doi.org/10.3389/fmicb.2015.00532
resource representing a document's title	Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens
has PubMed Central identifier	PMC4446546
has PubMed identifier	26074910
schema:publication	Front Microbiol
resource representing a document's body	covid:9223fd6478822761845291fe2ac429ca6ddbb45a#body_text
is schema:about of	named entity 'large number' named entity 'pathogens' named entity 'microarray' named entity 'OLD' named entity 'STAPHYLOCOCCUS ' named entity 'STUDY' named entity 'ONE OF' named entity 'MULTIPLE' named entity 'BROAD' named entity 'PATIENTS' named entity 'MYCOPLASMA PNEUMONIAE' named entity 'RESPONSIBLE' named entity 'AGED' named entity 'ASSOCIATION' named entity 'OUTBREAK' named entity 'ACTUAL' named entity 'BURDEN' named entity 'SPECTRUM' named entity 'INVESTIGATION' named entity 'DESCRIBED' named entity 'CONSECUTIVE' named entity 'QRT-PCR' named entity 'SIMULTANEOUS' named entity 'ESTABLISHED' named entity 'REAL-TIME PCR ' named entity 'STREPTOCOCCUS ' named entity 'NEW' named entity 'GENERAL HOSPITALS' named entity 'ASPIRATES' named entity '100%' named entity 'MONTH' named entity 'SEQUENCES' named entity 'YEAR' named entity 'STREPTOCOCCUS PNEUMONIAE' named entity 'USE OF' named entity 'ADDITIONAL' named entity 'EPIDEMIC' named entity 'KLEBSIELLA PNEUMONIAE' named entity 'LARGE' named entity 'TECHNIQUE' named entity 'DISCRIMINATION' named entity 'CONSISTENT WITH' named entity 'TOTAL' named entity 'GREAT' named entity 'BACTERIAL' named entity 'NUMBER OF' named entity 'COMPLEX' named entity 'YEARS' named entity 'RECENTLY' named entity 'MORAXELLA CATARRHALIS' named entity 'VIRAL AND' named entity 'FOUND' named entity 'MICROARRAY' named entity 'TARGETED' named entity 'HAEMOPHILUS' named entity 'ORGANISMS' named entity 'SAMPLES' named entity 'COMPARED' named entity 'DIAGNOSTICS' named entity 'PCR' named entity 'RESPIRATORY PATHOGENS' named entity 'IDENTIFICATION'

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