About: Microfluidic devices harboring unsealed reactors for real-time isothermal helicase-dependent amplification

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About: Microfluidic devices harboring unsealed reactors for real-time isothermal helicase-dependent amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Microfluidic devices harboring unsealed reactors for real-time isothermal helicase-dependent amplification
Creator	Tong, A Ae, Mak Gong, Hai-Qing Kai, Jin Matthew, A Mun, Yew Ramalingam, Naveen San, Chee Teo, A
topic	covid:91a672be533dd4bc35a9415bbef7470bc4ba3505#this
Source	PMC
abstract	High-throughput microchip devices used for nucleic-acid amplification require sealed reactors. This is to prevent evaporative loss of the amplification mixture and cross-contamination, which may occur among fluidically connected reactors. In most high-throughput nucleic-acid amplification devices, reactor sealing is achieved by microvalves. Additionally, these devices require micropumps to distribute amplification mixture into an array of reactors, thereby increasing the device cost, and adding complexity to the chip fabrication and operation processes. To overcome these limitations, we report microfluidic devices harboring open (unsealed) reactors in conjunction with a single-step capillary based flow scheme for sequential distribution of amplification mixture into an array of reactors. Concern about evaporative loss in unsealed reactors have been addressed by optimized reactor design, smooth internal reactor surfaces, and incorporation of a localized heating scheme for the reactors, in which isothermal, real-time helicase-dependent amplification (HDA) was performed.
has issue date	2009-01-09(xsd:dateTime)
bibo:doi	10.1007/s10404-008-0378-1
bibo:pmid	32214955
has license	no-cc
sha1sum (hex)	91a672be533dd4bc35a9415bbef7470bc4ba3505
schema:url	https://doi.org/10.1007/s10404-008-0378-1
resource representing a document's title	Microfluidic devices harboring unsealed reactors for real-time isothermal helicase-dependent amplification
has PubMed Central identifier	PMC7087983
has PubMed identifier	32214955
schema:publication	Microfluid Nanofluidics
resource representing a document's body	covid:91a672be533dd4bc35a9415bbef7470bc4ba3505#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/91a672be533dd4bc35a9415bbef7470bc4ba3505
is schema:about of	named entity 'isothermal' named entity 'scheme' named entity 'real-time' named entity 'Concern' named entity 'smooth' named entity 'devices' named entity 'reactors' named entity 'internal' named entity 'SEALING' named entity 'ADDING' named entity 'HARBORING' named entity 'OPERATION' named entity 'LIMITATIONS' named entity 'SEQUENTIAL' named entity 'MICROCHIP' named entity 'OPEN' named entity 'DEVICES USED' named entity 'mixture' named entity 'borosilicate glass' named entity 'nucleic-acid' named entity 'real-time PCR' named entity 'fluorescence resonance energy transfer' named entity 'MgSO' named entity 'isopropanol' named entity 'PDMS' named entity 'RNase H' named entity 'SVR' named entity 'isothermal' named entity 'PDMS' named entity 'non-specific' named entity 'isothermal' named entity 'PDMS' named entity 'PCR' named entity 'primers' named entity 'PDMS' named entity 'PCR' named entity 'RNase' named entity 'annealing' named entity 'amplicon' named entity 'adsorption' named entity 'microreactor' named entity 'plasmid DNA' named entity 'oligonucleotides' named entity 'Promega' named entity 'double-stranded DNA' named entity 'Pisa' named entity 'microreactor' named entity 'thermal cycling' named entity 'Transcription mediated amplification' named entity 'DNA-intercalating' named entity 'low-powered' named entity 'Agilent' named entity 'BSA' named entity 'Microcaps' named entity 'nucleic-acid' named entity 'polypropylene' named entity 'SVR' named entity 'PCR' named entity 'nucleic-acid' named entity 'SNP' named entity 'mixture' named entity 'Triton X-100' named entity 'PDMS' named entity 'PDMS' named entity '16.8' named entity 'gel electrophoresis' named entity 'reverse transcriptase' named entity 'PCR' named entity 'Triton X-100' named entity 'isothermal' named entity 'BSA'

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