About: Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region

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About: Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region
Creator	Yang, G Benson, R Brown, E Fields, B Pelish, T Winchell, J
source	Elsevier; Medline; PMC
abstract	Abstract Although the majority of cases of Legionnaires’ disease (LD) are caused by Legionella pneumophila, an increasing number of other Legionella species have been reported to cause human disease. There are no clinical presentations unique to LD and hence accurate laboratory tests are required for early diagnosis. Therefore, we designed a real-time PCR assay that targets the 23S-5S rRNA intergenic spacer region (23S-5S PCR) and allows for detection of all Legionella species and discrimination of L. pneumophila from other Legionella species. In total, 271 isolates representing 50 Legionella species were tested and the assay was validated using 39 culture-positive and 110 culture-negative patient specimens collected between 1989 and 2006. PCR-positive results were obtained with all 39 culture-positive samples (100% sensitivity). Specimens that tested positive according to 23S-5S PCR, but were culture-negative, were further analysed by DNA sequencing of the amplicon or the macrophage infectivity potentiator (mip) gene. In addition to L. pneumophila, Legionella longbeachae, Legionella cincinnatiensis and Legionella micdadei were identified in the specimens. The assay showed a 7-log dynamic range displaying a sensitivity of 7.5 CFU/mL or three genome equivalents per reaction. Sixty-one specimens containing viruses or bacteria other than Legionellae were negative according to 23S-5S PCR, demonstrating its specificity. Use of this assay should contribute to the earlier detection of respiratory disease caused by Legionella species, as well as to increased rates of detection.
has issue date	2010-03-31(xsd:dateTime)
bibo:doi	10.1111/j.1469-0691.2009.02766.x
bibo:pmid	19438641
has license	els-covid
sha1sum (hex)	913947b9629eb88d1777447affa49a1f45de7e46
schema:url	https://doi.org/10.1111/j.1469-0691.2009.02766.x
resource representing a document's title	Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region
has PubMed Central identifier	PMC7129662
has PubMed identifier	19438641
schema:publication	Clinical Microbiology and Infection
resource representing a document's body	covid:913947b9629eb88d1777447affa49a1f45de7e46#body_text
is schema:about of	named entity 'DETECTION' named entity 'There' named entity 'reaction' named entity 'number' named entity 'analysed' named entity 'collected' named entity 'Therefore' named entity 'viruses' named entity 'bacteria' named entity 'gene' named entity 'region' named entity 'targeting' named entity 'species' named entity 'gene' named entity 'COLLECTED' named entity 'RESULTS' named entity 'INCREASED RATES' named entity 'ACCURATE' named entity 'INCREASING' named entity 'CLINICAL' named entity 'BACTERIA ' named entity 'AMPLICON' named entity 'CAUSE' named entity 'SENSITIVITY' named entity 'CULTURE' named entity 'ISOLATES' named entity 'ALLOWS' named entity 'EARLY DIAGNOSIS' named entity 'REAL-TIME PCR ' named entity 'USING' named entity 'PCR ASSAY' named entity 'DETECTION' named entity 'NEGATIVE' named entity 'DISCRIMINATION' named entity 'EQUIVALENTS' named entity '5S RRNA' named entity 'TARGETS' named entity 'DISEASE' named entity 'MAJORITY' named entity 'MACROPHAGE' named entity 'USE OF' named entity 'GENE' named entity 'PNEUMOPHILA' named entity 'CFU' named entity 'A 7' named entity 'LEGIONELLA' named entity 'LEGIONELLA' named entity 'SPECIES' named entity 'ADDITION' named entity 'NUMBER OF' named entity 'RESPIRATORY DISEASE' named entity 'ITS' named entity '23S' named entity 'UNIQUE' named entity 'SAMPLES' named entity 'TESTED' named entity 'ASSAY' named entity 'REQUIRED' named entity 'VIRUSES' named entity 'DNA SEQUENCING' named entity 'OTHER THAN' named entity 'INFECTIVITY' named entity '110' named entity '7.5' named entity 'SPECIMENS' named entity 'PCR' named entity 'INTERGENIC SPACER' named entity 'HAVE' named entity 'IDENTIFIED' named entity 'CONTRIBUTE ' named entity 'OBTAINED' named entity 'LEGIONELLA PNEUMOPHILA' named entity 'SPECIFICITY' named entity 'TOTAL' named entity 'SPACER REGION' named entity '23S'

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