About: Isolation and characterization of Chinese porcine epidemic diarrhea virus with novel mutations and deletions in the S gene

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About: Isolation and characterization of Chinese porcine epidemic diarrhea virus with novel mutations and deletions in the S gene Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and characterization of Chinese porcine epidemic diarrhea virus with novel mutations and deletions in the S gene
Creator	Shi, Lin Fang, Weihuan Ma, Yuanmei Song, Houhui Ba, Shaobo He, Haijian Jiang, Sheng Li, Qunjing Shao, Chunyan Sun, Jing Wang, Xiaodu Wu, Yuan Zhou, Yingshan
Source	Elsevier; Medline; PMC
abstract	Porcine epidemic diarrhea (PEDV) has raised growing concerns in the pig-breeding industry because it has caused significant economic losses. To better understand the molecular epidemiology and genetic diversity of PEDV field isolates, in this study, the complete spike (S) and ORF3 genes of 17 PEDV variants in Zhejiang, China during 2014 to 2017, were characterized and analyzed. Phylogenetic analysis based on the S gene and ORF3 gene of these 17 novel PEDV strains and PEDV reference strains indicated that all the PEDV strains fell into two groups designated G1 and G2. Notably, the strains identified in 2014–2015 were in G2, while the other five strains identified from 2016 to 2017 were in G1. Sequencing and phylogenetic analyses showed that recently prevalent Chinese PEDV field strains shared higher identities with United States strains than with South Korean strains. Compared with classical vaccine strains, a series of deletions and frequently occurring mutations were observed in the receptor binding domains of our PEDV strains. Besides, we successfully isolated and reported the genetic characterization two novel PEDV strains, PEDV-LA1 and PEDV-LY4-98, found on the Chinese mainland, which had significant variations in the S gene. Meanwhile, the virulence of the new mutants may be changed, the PEDV-LY4-98 strain, which has multiple mutations in the signal peptide-encoding fragment of the S gene showed delayed cytopathic effects and smaller plaque size compared with strain PEDV-LA1, which lacks these mutations. Three unique amino acid substitutions (L7, G8, and V9) were identified in the SP-encoding fragment of the S1 N-terminal domain of the PEDV-LY4-98 S protein compared with the S proteins of all the previous PEDV strains. The animal experiment revealed that these two novel strains were high pathogenic to neonatal pigs. Whether these amino acids substitutions and the N-glycosylation site substitutions influence the antigenicity and pathogenicity of PEDV remains to be investigated. Meanwhile, amino acid substitutions in the neutralizing epitopes may have conferred the capacity for immune evasion in these PEDV field strains. This study improves our understanding of ongoing PEDV outbreaks in China, and it will guide further efforts to develop effective measures to control this virus.
has issue date	2018-06-06(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2018.05.021
bibo:pmid	29981713
has license	no-cc
sha1sum (hex)	9055c4e8d4511d973e2246580fc1184557946012
schema:url	https://doi.org/10.1016/j.vetmic.2018.05.021
resource representing a document's title	Isolation and characterization of Chinese porcine epidemic diarrhea virus with novel mutations and deletions in the S gene
has PubMed Central identifier	PMC7117340
has PubMed identifier	29981713
schema:publication	Vet Microbiol
resource representing a document's body	covid:9055c4e8d4511d973e2246580fc1184557946012#body_text
is schema:about of	named entity 'strain' named entity 'vaccine' named entity 'groups' named entity 'substitutions' named entity 'United States' named entity 'Isolation' named entity 'deletions' named entity 'MUTATIONS' named entity 'NOVEL' named entity 'LACKS' named entity 'GROWING' named entity 'N-GLYCOSYLATION SITE' named entity 'UNDERSTANDING' named entity 'CHARACTERIZED' named entity 'STRAINS' named entity 'OBSERVED' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'FOUND' named entity 'SEQUENCING' named entity 'FIELD' named entity 'AMINO ACID SUBSTITUTIONS' named entity 'SIZE' named entity 'PATHOGENICITY' named entity 'HIGH' named entity 'IMMUNE EVASION' named entity 'GENETIC DIVERSITY' named entity 'UNITED STATES' named entity 'CAPACITY' named entity 'SMALLER' named entity 'GENETIC CHARACTERIZATION' named entity 'CONCERNS' named entity 'ISOLATES' named entity 'COMPARED' named entity 'INVESTIGATED' named entity 'GENE' named entity 'INDICATED' named entity 'HIGHER' named entity 'CHARACTERIZATION' named entity 'KOREAN' named entity 'BASED' named entity 'PLAQUE' named entity 'BREEDING' named entity 'MAY BE' named entity 'SERIES' named entity 'ECONOMIC' named entity 'ONGOING' named entity 'NEONATAL' named entity 'UNIQUE' named entity 'STUDY' named entity 'COMPLETE' named entity 'FELL' named entity 'MULTIPLE' named entity 'PREVIOUS' named entity 'IDENTITIES' named entity 'INFLUENCE' named entity 'INDUSTRY' named entity 'CYTOPATHIC EFFECTS' named entity 'TO CONTROL' named entity 'GROUPS' named entity 'ISOLATED' named entity 'EPITOPES' named entity '28S' named entity 'VACCINE' named entity 'S PROTEIN' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'SPIKE' named entity 'PROTEINS' named entity 'PEDV' named entity 'OUR'

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