About: SARS-coronavirus spike S2 domain flanked by cysteine residues C822 and C833 is important for activation of membrane fusion

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About: SARS-coronavirus spike S2 domain flanked by cysteine residues C822 and C833 is important for activation of membrane fusion Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-coronavirus spike S2 domain flanked by cysteine residues C822 and C833 is important for activation of membrane fusion
Creator	Belouzard, Sandrine Madu, Ikenna Whittaker, Gary
Source	Elsevier; Medline; PMC
abstract	Abstract The S2 domain of the coronavirus spike (S) protein is known to be responsible for mediating membrane fusion. In addition to a well-recognized cleavage site at the S1–S2 boundary, a second proteolytic cleavage site has been identified in the severe acute respiratory syndrome coronavirus (SARS-CoV) S2 domain (R797). C-terminal to this S2 cleavage site is a conserved region flanked by cysteine residues C822 and C833. Here, we investigated the importance of this well conserved region for SARS-CoV S-mediated fusion activation. We show that the residues between C822–C833 are well conserved across all coronaviruses. Mutagenic analysis of SARS-CoV S, combined with cell–cell fusion and pseudotyped virion infectivity assays, showed a critical role for the core-conserved residues C822, D830, L831, and C833. Based on available predictive models, we propose that the conserved domain flanked by cysteines 822 and 833 forms a loop structure that interacts with components of the SARS-CoV S trimer to control the activation of membrane fusion.
has issue date	2009-10-25(xsd:dateTime)
bibo:doi	10.1016/j.virol.2009.07.038
bibo:pmid	19717178
has license	els-covid
sha1sum (hex)	8f66fa419ca3a45f932b729bac2a50e355c65203
schema:url	https://doi.org/10.1016/j.virol.2009.07.038
resource representing a document's title	SARS-coronavirus spike S2 domain flanked by cysteine residues C822 and C833 is important for activation of membrane fusion
has PubMed Central identifier	PMC3594805
has PubMed identifier	19717178
schema:publication	Virology
resource representing a document's body	covid:8f66fa419ca3a45f932b729bac2a50e355c65203#body_text
is schema:about of	named entity 'Here' named entity 'structure' named entity 'site' named entity 'activation' named entity 'severe acute respiratory syndrome coronavirus' named entity 'MUTAGENIC' named entity 'IS A' named entity 'DOMAIN' named entity 'MEDIATED' named entity 'CONSERVED' named entity 'SPIKE' named entity 'HERE' named entity 'IDENTIFIED' named entity 'MEDIATING' named entity 'ACTIVATION' named entity 'CLEAVAGE' named entity 'CYSTEINE' named entity 'CONSERVED REGION' named entity 'SITE' named entity 'AVAILABLE' named entity 'ADDITION' named entity 'FUSION' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'FLANKED' named entity 'CYSTEINE' named entity 'DOMAIN' named entity 'SPIKE' named entity 'SARS' named entity 'IMPORTANT' named entity 'PROTEOLYTIC' named entity 'COMPONENTS' named entity 'ANALYSIS' named entity 'PROTEIN ' named entity 'CORONAVIRUSES' named entity 'CRITICAL' named entity 'KNOWN' named entity 'TO CONTROL' named entity 'C-TERMINAL' named entity '28S' named entity 'VIRION' named entity 'FLANKED' named entity 'SECOND' named entity 'PREDICTIVE MODELS' named entity 'CELL-CELL FUSION' named entity 'BOUNDARY' named entity 'TRIMER' named entity 'FORMS' named entity 'INVESTIGATED' named entity 'CORE' named entity 'ACROSS' named entity 'CORONAVIRUS' named entity 'MEMBRANE FUSION' named entity 'COMBINED' named entity 'MEMBRANE FUSION' named entity '833' named entity 'ROLE' named entity 'BASED' named entity 'LOOP STRUCTURE' named entity 'SARS-COV' named entity 'ACTIVATION' named entity 'CORONAVIRUS' named entity 'INFECTIVITY' named entity 'RESPONSIBLE' named entity 'SARS-CoV' named entity 'loop' named entity 'cysteine' named entity 'site' named entity 'predictive models' named entity 'domain' named entity 'conserved region' named entity 'trimer' named entity 'cell-cell' named entity 'cleavage site' named entity 'SARS-CoV' named entity 'critical role' named entity 'infectivity' named entity 'C-terminal' named entity 'cleavage site' named entity 'Vero E6'

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