About: CRISPR-Assisted DNA Detection, a novel dCas9-based DNA detection technique

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	CRISPR-Assisted DNA Detection, a novel dCas9-based DNA detection technique
Creator	Li, Weiwei Lin, Na Luo, Tao Xia, Xinyi Gao, Jinliang Wang, Jinke Xu, Xinhui
Source	BioRxiv
abstract	Nucleic acid detection techniques are always critical to diagnosis, especially in the background of the present COVID-19 pandemic. The simple and rapid detection techniques with high sensitivity and specificity are always urgently needed. However, the current nucleic acid detection techniques are still limited the traditional amplification and hybridization. To overcome the limitation, we here develop a CRISPR/Cas9-assisted DNA detection (CADD). In this detection, DNA sample is incubated with a pair of capture sgRNAs (sgRNAa and sgRNAb) specific to a target DNA, dCas9, a signal readout-related probe, and an oligo-coated solid support beads or microplate at room temperature for 15 min. During this incubation, the dCas9-sgRNA-DNA complex is formed and captured on solid support by the capture sequence of sgRNAa and the signal readout-related probe is captured by the capture sequence of sgRNAb. Finally the detection result is reported by a fluorescent or colorimetric signal readout. This detection was verified by detecting DNA of bacteria, cancer cell and virus. Especially, by designing a set of sgRNAs specific to 15 high-risk human papillomaviruses (HPVs), the HPV infection in 64 clinical cervical samples were successfully detected by the method. All detections can be finished in 30 minutes at room temperature. This detection holds promise for rapid on-the-spot detection or point-of-care testing (POCT).
has issue date	2020-05-13(xsd:dateTime)
bibo:doi	10.1101/2020.05.13.093062
has license	biorxiv
sha1sum (hex)	8a87819d0e8ec3688e423ae0d34af3e4b45f7374
schema:url	https://doi.org/10.1101/2020.05.13.093062
resource representing a document's title	CRISPR-Assisted DNA Detection, a novel dCas9-based DNA detection technique
schema:publication	bioRxiv
resource representing a document's body	covid:8a87819d0e8ec3688e423ae0d34af3e4b45f7374#body_text
is schema:about of	named entity 'captured' named entity 'DNA' named entity 'pandemic' named entity 'solid' named entity 'hybridization' covid:arg/8a87819d0e8ec3688e423ae0d34af3e4b45f7374 named entity 'detection' named entity 'beads' named entity 'sample' named entity 'specific' named entity 'captured' named entity 'DNA' named entity 'room temperature' named entity 'background' named entity '15 min' named entity 'Cas9' named entity 'sgRNA' named entity 'incubation' named entity 'COVID-19 pandemic' named entity 'oligo' named entity 'CRISPR' named entity 'rapid' named entity 'DNA samples' named entity 'single nucleotide polymorphisms' named entity 'oligo' named entity 'sensitivity and specificity' named entity 'ASFV' named entity 'Microplate' named entity 'oligo' named entity 'dCas9' named entity 'S4D' named entity 'CRISPR' named entity 'RPA' named entity 'promoter DNA' named entity 'streptavidin' named entity 'transcription' named entity 'Cas' named entity 'TERT' named entity 'oligo' named entity 'HeLa' named entity 'dCas9' named entity 'RPA' named entity 'carcinogenic' named entity 'cervical cancer' named entity 'Takara' named entity 'HPV' named entity 'double-stranded DNA virus' named entity 'point-of-care testing' named entity 'gDNA' named entity 'China' named entity 'agarose gel electrophoresis' named entity 'protein' named entity 'DNA sample' named entity 'S4D' named entity 'oligo' named entity 'Cas9' named entity 'African swine fever virus' named entity 'dCas9' named entity 'plasmids' named entity 'Hairpin' named entity 'microplate reader' named entity 'primers' named entity 'virus' named entity 'Cas12a' named entity 'biotin' named entity 'dCas9' named entity 'telomerase' named entity 'gDNA' named entity 'cancer cell lines' named entity 'wild-type' named entity 'TERT' named entity 'dCas9' named entity 'HPV' named entity 'TERT' named entity 'oligonucleotide' named entity 'human papillomaviruses' named entity 'T7 RNA polymerase' named entity 'binding complex'

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