About: Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques
Creator	Wark, Peter Gibson, Peter Johnston, Sebastian Simpson, Jodie Moric, Ivana
Source	Elsevier; Medline; PMC
abstract	Background: Influenza (Flu) and respiratory syncytial virus (RSV) are important viral pathogens that cause lower respiratory tract infections and severe exacerbations of asthma. Molecular biological techniques are permitting a rapid and accurate diagnosis of infections caused by respiratory pathogens, and have typically been applied to upper respiratory samples. Sputum induction provides an opportunity to directly sample secretions from the lower respiratory tract. Objectives/study design: To determine the role of induced sputum reverse-transcription polymerase chain reaction (RT-PCR) in the detection of respiratory pathogens and compare this with detection using serology and immunofluorescent antigen (IFA) testing, we recruited 49 adults from emergency room with exacerbations of asthma. After a medical assessment and spirometry, sputum was induced using ultrasonically nebulised normal saline. Sputum was assayed using IFA and RT-PCR for flu and RSV. Flu serology was performed acutely and at convalescence, 4–5 weeks later. Results: Influenza A or B was detected in 24% of the samples by PCR, significantly more than the nine cases detected using serology and the one case using IFA (P<0.05). RSV was detected in 37% of samples using PCR and 20% by IFA (P<0.05). Conclusion: The combination of induced sputum and RT-PCR provides a useful means of detecting respiratory infection. The technique is safe in both adults and children, and RT-PCR is more sensitive than conventional serology and IFA. The improved sensitivity of induced sputum RT-PCR also permits a more rapid diagnosis and the opportunity of early administration of effective treatments.
has issue date	2002-10-23(xsd:dateTime)
bibo:doi	10.1016/s1386-6532(02)00084-7
bibo:pmid	12637083
has license	no-cc
sha1sum (hex)	85d6ee282a18e659c53f5fc532668ee72e68b8ef
schema:url	https://doi.org/10.1016/s1386-6532%2802%2900084-7
resource representing a document's title	Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques
has PubMed Central identifier	PMC7270923
has PubMed identifier	12637083
schema:publication	J Clin Virol
resource representing a document's body	covid:85d6ee282a18e659c53f5fc532668ee72e68b8ef#body_text
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