About: Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics

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About: Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics
Creator	Yu, Lan Wang, Yong Fu, Yu Zhang, Xiaoli Bao, Mingjia Li, Chunjiang Su, Shanshan Wang, Yuchao Zhang, Jisheng Zhao, Yongxin
Source	PMC
abstract	BACKGROUND: Carbapenem-resistant hypervirulent Klebsiella pneumoniae strains have recently come into existence worldwide; however, researchers in northeast China are not aware of their clinical features and molecular characteristics. METHODS: Here, the molecular and virulent characteristics of 44 carbapenem-resistant K. pneumoniae (CRKP) isolates collected from January 2015 to December 2017 were studied. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were carried out to define the clonal relatedness among the isolates. PCR and capsular serotyping of the virulence-associated genes, as well as biofilm formation and serum complement-mediated killing assays, were employed to determine the virulent potential. The genomic features and associated mobile genetic elements of JmsCRE57 were detected by whole genome sequencing. RESULTS: The only positive isolate was JmsCRE57, which belonged to the ST375 serotype K2 that expressed uge, mrkD, fimH, kpn, aerobactin and rmpA virulence-associated genes and showed strong biofilm formation and serum sensitivity. Sequencing results showed that the JmsCRE57 genome mainly consisted of a circular chromosome, three antimicrobial resistant plasmids and a virulent plasmid. The antimicrobial resistant plasmid expressing bla(KPC-2), bla(CTX-M-15), aph(3″)-Ib, aph(6)-Id, qnrB1, aac(3)-IIa, aac(6′)-Ib-cr, bla(OXA-1), bla(TEM-1B), catB4, sul2, dfrA14 and bla(SHV-99). The virulent plasmid belonged to the IncHI1B group, which is mainly composed of mucoid phenotype genes and siderophore-associated genes. The remaining CRKP strains that expressed uge, fimH, mrkD and kpn virulence-associated genes were not successfully typed. CONCLUSION: Our results provide new insights on the epidemiology of carbapenem-resistant K2 hypervirulent K. pneumoniae ST375 and CRKP ST76 strains in northeast China, which may help control their future outbreaks.
has issue date	2020-07-02(xsd:dateTime)
bibo:doi	10.1186/s12879-020-05143-y
bibo:pmid	32616018
has license	cc-by
sha1sum (hex)	841980902bbe37d7053331bd99a96d519dd38ab5
schema:url	https://doi.org/10.1186/s12879-020-05143-y
resource representing a document's title	Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics
has PubMed Central identifier	PMC7331116
has PubMed identifier	32616018
schema:publication	BMC Infect Dis
resource representing a document's body	covid:841980902bbe37d7053331bd99a96d519dd38ab5#body_text
is schema:about of	named entity 'northeast China' named entity 'Northeast China' named entity 'Klebsiella pneumoniae' named entity 'hypervirulent' named entity 'clinical features' named entity 'virulent' named entity 'plasmid' named entity 'K. pneumoniae' named entity 'agarose gel' named entity 'fimbriae' named entity 'biofilm formation' named entity 'abscesses' named entity 'paired-end' named entity 'serotypes' named entity 'virulent' named entity 'plasmid' named entity 'kpn' named entity 'quinolone' named entity 'virulence' named entity 'ceftriaxone' named entity 'antimicrobial resistance' named entity 'TEM' named entity 'GC content' named entity 'biofilm' named entity 'Nanchang' named entity 'microplate reader' named entity 'K. pneumoniae' named entity 'resistance gene' named entity 'absorbance' named entity 'trimethoprim' named entity 'carbapenem' named entity 'Venous blood' named entity 'aminoglycosides' named entity 'CTX-M' named entity 'biofilm formation' named entity 'ATCC' named entity 'locus' named entity 'alleles' named entity 'complement' named entity 'imipenem' named entity 'antimicrobials' named entity 'carbapenemases' named entity 'MLST' named entity 'CRKP' named entity 'KPC' named entity 'CRKP' named entity 'hypervirulent' named entity 'significant difference' named entity 'KPC' named entity 'epidemiological surveillance' named entity 'refractory' named entity 'MICs' named entity 'Multidrug resistant' named entity 'PBS' named entity 'bacteria' named entity 'viscous' named entity 'antimicrobials' named entity 'gene expression' named entity 'siderophores' named entity 'CRKP' named entity 'serum' named entity 'serotype' named entity 'phenotype' named entity 'gene' named entity 'hematology' named entity 'streptomycin' named entity 'plasmid' named entity 'transposon' named entity 'fosfomycin' named entity 'Virulence' named entity 'genome' named entity 'Klebsiella pneumoniae'

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