About: Epithelial cell lines of the cotton rat (Sigmodon hispidus) are highly susceptible in vitro models to zoonotic Bunya-, Rhabdo-, and Flaviviruses

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About: Epithelial cell lines of the cotton rat (Sigmodon hispidus) are highly susceptible in vitro models to zoonotic Bunya-, Rhabdo-, and Flaviviruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Epithelial cell lines of the cotton rat (Sigmodon hispidus) are highly susceptible in vitro models to zoonotic Bunya-, Rhabdo-, and Flaviviruses
Creator	Drosten, Christian Eckerle, Isabella Specht, Sabine Junglen, Sandra Ehlen, Lukas Kallies, René Papies, Jan Tödtmann, Jan Müller, Marcel
Source	Medline; PMC
abstract	BACKGROUND: Small mammals such as bats and rodents have been increasingly recognized as reservoirs of novel potentially zoonotic pathogens. However, few in vitro model systems to date allow assessment of zoonotic viruses in a relevant host context. The cotton rat (Sigmodon hispidus) is a New World rodent species that has a long-standing history as an experimental animal model due to its unique susceptibility to human viruses. Furthermore, wild cotton rats are associated with a large variety of known or potentially zoonotic pathogens. METHODS: A method for the isolation and culture of airway epithelial cell lines recently developed for bats was applied for the generation of rodent airway and renal epithelial cell lines from the cotton rat. Continuous cell lines were characterized for their epithelial properties as well as for their interferon competence. Susceptibility to members of zoonotic Bunya-, Rhabdo-, and Flaviviridae, in particular Rift Valley fever virus (RVFV), vesicular stomatitis virus (VSV), West Nile virus (WNV), and tick-borne encephalitis virus (TBEV) was tested. Furthermore, novel arthropod-derived viruses belonging to the families Bunya-, Rhabdo-, and Mesoniviridae were tested. RESULTS: We successfully established airway and kidney epithelial cell lines from the cotton rat, and characterized their epithelial properties. Cells were shown to be interferon-competent. Viral infection assays showed high-titre viral replication of RVFV, VSV, WNV, and TBEV, as well as production of infectious virus particles. No viral replication was observed for novel arthropod-derived members of the Bunya-, Rhabdo-, and Mesoniviridae families in these cell lines. CONCLUSION: In the current study, we showed that newly established cell lines from the cotton rat can serve as host-specific in vitro models for viral infection experiments. These cell lines may also serve as novel tools for virus isolation, as well as for the investigation of virus-host interactions in a relevant host species.
has issue date	2016-05-04(xsd:dateTime)
bibo:doi	10.1186/s12985-016-0531-5
bibo:pmid	27142375
has license	cc-by
sha1sum (hex)	7fdea1d57d5d3d0ee393379c8437b0393f0ce542
schema:url	https://doi.org/10.1186/s12985-016-0531-5
resource representing a document's title	Epithelial cell lines of the cotton rat (Sigmodon hispidus) are highly susceptible in vitro models to zoonotic Bunya-, Rhabdo-, and Flaviviruses
has PubMed Central identifier	PMC4855710
has PubMed identifier	27142375
schema:publication	Virol J
resource representing a document's body	covid:7fdea1d57d5d3d0ee393379c8437b0393f0ce542#body_text
is schema:about of	named entity 'variety' named entity 'KNOWN' named entity 'UNIQUE' named entity 'ASSOCIATED WITH' named entity 'NOVEL' named entity 'IS A' named entity 'RESERVOIRS' named entity 'LARGE' named entity 'HOST' named entity 'ITS' named entity 'NEW' named entity 'VIRUSES' named entity 'HAVE' named entity 'HISTORY' named entity 'IN VITRO' named entity 'MAMMALS' named entity 'COTTON RAT' named entity 'WORLD' named entity 'RODENTS' named entity 'RELEVANT' named entity 'SMALL' named entity 'WILD' named entity 'HUMAN' named entity 'ASSESSMENT' named entity 'DUE TO' named entity 'SYSTEMS' named entity 'ZOONOTIC PATHOGENS' named entity 'SUSCEPTIBILITY' named entity 'VARIETY' named entity 'MODELS' named entity 'SPECIES' named entity 'BATS' named entity 'RODENT' named entity 'ABSTRACT' named entity 'COTTON RATS' named entity 'CELL LINES' named entity 'EXPERIMENTAL ANIMAL MODEL' named entity 'IN VITRO MODEL' named entity 'CONTEXT' named entity 'SUSCEPTIBLE' named entity 'ALLOW' named entity 'HIGHLY' named entity 'BACKGROUND' named entity 'EPITHELIAL CELL' named entity 'ZOONOTIC' named entity 'FLAVIVIRUSES' named entity 'DATE' covid:arg/7fdea1d57d5d3d0ee393379c8437b0393f0ce542 named entity 'history' named entity 'However' named entity 'zoonotic' named entity 'cotton rat' named entity 'large' named entity 'context' named entity 'Small' named entity 'reservoirs' named entity 'model systems' named entity 'Small mammals' named entity 'Rhabdo' named entity 'animal model' named entity 'zoonotic pathogens' named entity 'zoonotic pathogens' named entity 'New World' named entity 'Sigmodon hispidus' named entity 'Flaviviruses' named entity 'Sigmodon hispidus' named entity 'cotton rat' named entity 'Dulbecco's modified Eagle's medium' named entity 'viral replication' named entity 'infection' named entity 'virus' named entity 'viral RNA' named entity 'VSV'

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