About: Binding interaction of SARS coronavirus 3CL(pro) protease with vacuolar-H(+) ATPase G1 subunit

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About: Binding interaction of SARS coronavirus 3CL(pro) protease with vacuolar-H(+) ATPase G1 subunit Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Binding interaction of SARS coronavirus 3CL(pro) protease with vacuolar-H(+) ATPase G1 subunit
Creator	Lin, Cheng-Wen Wan, Lei Lin, Kuan-Hsun Hsieh, Tsung-Han Lai, Chien-Chen Li, Jeng-Yi Shiu, Shi-Yi Tsai, Fuu-Jen
Source	Elsevier; Medline; PMC
abstract	The pathogenesis of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important issue for treatment and prevention of SARS. Recently, SARS-CoV 3CL(pro) protease has been implied to be possible relevance to SARS-CoV pathogenesis. In this study, we intended to identify potential 3CL(pro)-interacting cellular protein(s) using the phage-displayed human lung cDNA library. The vacuolar-H(+) ATPase (V-ATPase) G1 subunit that contained a 3CL(pro) cleavage site-like motif was identified as a 3CL(pro)-interacting protein, as confirmed using the co-immunoprecipitation assay and the relative affinity assay. In addition, our result also demonstrated the cleavage of the V-ATPase G1 fusion protein and the immunoprecipitation of cellular V-ATPase G1 by the 3CL(pro). Moreover, loading cells with SNARF-1 pH-sensitive dye showed that the intracellular pH in 3CL(pro)-expressing cells was significantly lower as compared to mock cells.
has issue date	2005-11-07(xsd:dateTime)
bibo:doi	10.1016/j.febslet.2005.09.075
bibo:pmid	16226257
has license	no-cc
sha1sum (hex)	7f796fc1f34c8e45bc773c7f0f7f982b750f7957
schema:url	https://doi.org/10.1016/j.febslet.2005.09.075
resource representing a document's title	Binding interaction of SARS coronavirus 3CL(pro) protease with vacuolar-H(+) ATPase G1 subunit
has PubMed Central identifier	PMC7094641
has PubMed identifier	16226257
schema:publication	FEBS Lett
resource representing a document's body	covid:7f796fc1f34c8e45bc773c7f0f7f982b750f7957#body_text
is schema:about of	named entity 'BINDING' named entity 'interacting' named entity 'assay' named entity 'loading' named entity 'SARS-CoV' named entity 'protease' named entity 'PH-SENSITIVE' named entity 'USING' named entity 'POSSIBLE' named entity 'CO-IMMUNOPRECIPITATION ASSAY' named entity 'PROTEIN' named entity 'MOTIF' named entity 'PREVENTION OF' named entity 'RELEVANCE' named entity 'CLEAVAGE' named entity 'CONTAINED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'DYE' named entity 'RESULT' named entity 'SARS' named entity 'PHAGE' named entity 'COMPARED' named entity 'SARS-COV' named entity 'LIKE' named entity 'HUMAN LUNG' named entity 'LOWER' named entity 'IMPORTANT' named entity 'PRO' named entity 'PATHOGENESIS' named entity 'RECENTLY' named entity 'V-ATPASE' named entity 'POTENTIAL' named entity 'INTRACELLULAR PH' named entity 'INTERACTING' named entity 'ISSUE' named entity 'TO IDENTIFY' covid:arg/7f796fc1f34c8e45bc773c7f0f7f982b750f7957 named entity 'INTERACTION' named entity 'INTENDED' named entity 'CONFIRMED' named entity 'PRO' named entity 'SUBUNIT' named entity 'ATPASE' named entity 'PROTEASE' named entity 'VACUOLAR' named entity 'ATPASE' named entity 'TREATMENT' named entity 'FUSION PROTEIN' named entity 'PROTEIN ' named entity 'SNARF-1' named entity 'CDNA LIBRARY' named entity 'ASSAY' named entity 'IMMUNOPRECIPITATION' named entity 'OUR' named entity 'STUDY' named entity '28S' named entity 'PROTEASE' named entity 'RELATIVE' named entity 'DISPLAYED' named entity 'CELLULAR' named entity 'SITE' named entity 'IDENTIFIED' named entity 'MOCK' named entity 'SUBUNIT' named entity 'CELLS' named entity 'LOADING' named entity 'SARS CORONAVIRUS' named entity 'VACUOLAR' named entity 'AFFINITY' named entity 'ADDITION' named entity 'cleavage' named entity 'cellular' named entity 'confirmed' named entity 'cells'

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