About: Using DNA Shuffling to Create Novel Infectious Bronchitis Virus S1 Genes: Implications for S1 Gene Recombination

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About: Using DNA Shuffling to Create Novel Infectious Bronchitis Virus S1 Genes: Implications for S1 Gene Recombination Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Using DNA Shuffling to Create Novel Infectious Bronchitis Virus S1 Genes: Implications for S1 Gene Recombination
Creator	Jackwood, Mark Callison, Scott Hilt, Deborah
Source	Medline; PMC
abstract	We employed the staggered extension process (StEP) to shuffle the S1 genes from four infectious bronchitis virus (IBV) strains representing four unique serotypes. Upon creating a shuffled S1 gene library, we randomly selected 25 clones and analyzed them by DNA sequencing. In total, eleven clones contained novel S1 gene recombinants. Based on sequence data, each recombinant was unique and contained a full-length open reading frame. The average number of crossovers per recombinant was 5 and the average number of point mutations was 1.3, leading mostly to non-synonymous amino acid changes. No recombinant contained sequences from all four parental genes and no recombinant contained any sequence from the distantly related Delaware 072 strain. Our data suggests that recombination between distantly related IBV strains within the S1 gene probably does not readily occur. This finding is extremely important in light of the common industry vaccination practice of mixing different live-attenuated IBV strains.
has issue date	2005-01-01(xsd:dateTime)
bibo:doi	10.1007/s11262-004-2194-3
bibo:pmid	15965603
has license	no-cc
sha1sum (hex)	7de78e86d6284601e15735bd2276edb8e46f606b
schema:url	https://doi.org/10.1007/s11262-004-2194-3
resource representing a document's title	Using DNA Shuffling to Create Novel Infectious Bronchitis Virus S1 Genes: Implications for S1 Gene Recombination
has PubMed Central identifier	PMC7088642
has PubMed identifier	15965603
schema:publication	Virus Genes
resource representing a document's body	covid:7de78e86d6284601e15735bd2276edb8e46f606b#body_text
is schema:about of	named entity 'unique' named entity 'strain' named entity 'industry' named entity 'Recombination' named entity 'S1 GENE' named entity 'NUMBER OF' named entity 'MIXING' named entity 'DNA SEQUENCING' named entity '072' named entity 'EXTREMELY IMPORTANT' named entity 'OPEN READING FRAME' named entity 'FINDING' named entity 'OCCUR' named entity 'BASED' named entity 'POINT MUTATIONS' named entity 'RECOMBINANT' named entity 'DATA' named entity 'OUR' named entity 'CREATE' named entity 'EXTENSION' named entity 'CONTAINED' named entity 'PRACTICE' named entity 'ANALYZED' named entity 'NON-' named entity 'RECOMBINATION' named entity 'SELECTED' named entity 'SEQUENCE' named entity 'EMPLOYED' named entity 'DOES NOT' named entity 'SEROTYPES' named entity '1.3' named entity 'PARENTAL' named entity 'STAGGERED' named entity 'GENES' named entity 'AMINO ACID' named entity 'VACCINATION' named entity 'CLONES' named entity 'CREATING' named entity 'STRAIN' named entity 'LEADING' named entity 'SEQUENCES' named entity 'LIGHT' named entity 'RELATED' named entity 'PROBABLY' named entity 'RECOMBINANTS' named entity 'ATTENUATED' named entity 'NOVEL' named entity 'DISTANTLY' named entity 'NOVEL' named entity 'USING' named entity 'GENES' named entity 'RECOMBINATION' named entity 'LIVE' named entity 'AVERAGE' named entity 'DIFFERENT' named entity 'DELAWARE' named entity 'COMMON' named entity 'SHUFFLE' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'INDUSTRY' named entity 'SYNONYMOUS' named entity 'SEQUENCE DATA' named entity 'TOTAL' named entity 'CHANGES' named entity 'UNIQUE' named entity 'STRAINS' named entity 'S1 GENE' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'DNA SHUFFLING' named entity 'GENE LIBRARY' named entity 'PROCESS' covid:arg/7de78e86d6284601e15735bd2276edb8e46f606b named entity 'clones' named entity 'gene' named entity 'Our' named entity 'virus' named entity 'sequences' named entity 'data' named entity 'contained'

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