About: Analysis of Qa-1(b)Peptide Binding Specificity and the Capacity of Cd94/Nkg2a to Discriminate between Qa-1

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Analysis of Qa-1(b)Peptide Binding Specificity and the Capacity of Cd94/Nkg2a to Discriminate between Qa-1–Peptide Complexes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Analysis of Qa-1(b)Peptide Binding Specificity and the Capacity of Cd94/Nkg2a to Discriminate between Qa-1–Peptide Complexes
Creator	Vance, Russell Jensen, Peter Martin, Amy Kraft, Jennifer Pohl, Jan Raulet, David
Source	PMC
abstract	The major histocompatibility complex class Ib protein, Qa-1(b), serves as a ligand for murine CD94/NKG2A natural killer (NK) cell inhibitory receptors. The Qa-1(b) peptide-binding site is predominantly occupied by a single nonameric peptide, Qa-1 determinant modifier (Qdm), derived from the leader sequence of H-2D and L molecules. Five anchor residues were identified in this study by measuring the peptide-binding affinities of substituted Qdm peptides in experiments with purified recombinant Qa-1(b). A candidate peptide-binding motif was determined by sequence analysis of peptides eluted from Qa-1 that had been folded in the presence of random peptide libraries or pools of Qdm derivatives randomized at specific anchor positions. The results indicate that Qa-1(b) can bind a diverse repertoire of peptides but that Qdm has an optimal primary structure for binding Qa-1(b). Flow cytometry experiments with Qa-1(b) tetramers and NK target cell lysis assays demonstrated that CD94/NKG2A discriminates between Qa-1(b) complexes containing peptides with substitutions at nonanchor positions P4, P5, or P8. Our findings suggest that it may be difficult for viruses to generate decoy peptides that mimic Qdm and raise the possibility that competitive replacement of Qdm with other peptides may provide a novel mechanism for activation of NK cells.
has issue date	2000-09-05(xsd:dateTime)
bibo:pmid	10974028
has license	cc-by-nc-sa
sha1sum (hex)	7ce952ec1f791dfa670858fa78a25863df0c24b2
resource representing a document's title	Analysis of Qa-1(b)Peptide Binding Specificity and the Capacity of Cd94/Nkg2a to Discriminate between Qa-1–Peptide Complexes
has PubMed Central identifier	PMC2193274
has PubMed identifier	10974028
schema:publication	J Exp Med
resource representing a document's body	covid:7ce952ec1f791dfa670858fa78a25863df0c24b2#body_text
is schema:about of	named entity 'study' named entity 'lysis' named entity 'motif' named entity 'peptide' named entity 'activation' named entity 'possibility' named entity 'mechanism' named entity 'Peptide' named entity 'Five' named entity 'affinities' named entity 'peptides' named entity 'tetramers' named entity 'eluted' named entity 'peptide' named entity 'peptides' named entity 'bind' named entity 'competitive' named entity 'peptides' named entity 'Flow cytometry' named entity 'anchor' named entity 'peptide' named entity 'leader sequence' named entity 'viruses' named entity 'peptide' named entity 'binding affinities' named entity 'CD94' named entity 'primary structure' named entity 'binding motif' named entity 'peptides' named entity 'murine' named entity 'CD94' named entity 'salts' named entity 'virus' named entity 'effector cells' named entity 'CD8' named entity 'leader peptide' named entity 'peptide' named entity 'HLA-G' named entity 'NK cells' named entity 'Cys' named entity 'EBV' named entity 'CD8' named entity 'sequence comparison' named entity 'lysis' named entity 'crystal structure' named entity 'C57BL/6' named entity 'peptide' named entity 'peptide' named entity 'mice' named entity 'Emory University' named entity 'deprotection' named entity 'Mtr' named entity 'peptide' named entity 'eluted' named entity 'peptide' named entity 'IL-2' named entity 'IL-2' named entity 'Electrospray ionization' named entity 'CD94' named entity 'CD94' named entity 'peptide' named entity 'exogenous' named entity 'sequence variation' named entity 'antigens' named entity 'HLA-E' named entity 'Trp' named entity 'IL-2' named entity 'acylation' named entity 'crystal structure' named entity 'infection' named entity 'mice' named entity 'transfected' named entity 'fluorometer' named entity 'phosphate buffer' named entity 'NKG2' named entity 'NK1.1' named entity 'lysis' named entity 'CD94'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software