About: Loop-mediated isothermal amplification method for rapid detection of the toxic dinoflagellate Alexandrium, which causes algal blooms and poisoning of shellfish

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About: Loop-mediated isothermal amplification method for rapid detection of the toxic dinoflagellate Alexandrium, which causes algal blooms and poisoning of shellfish Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Loop-mediated isothermal amplification method for rapid detection of the toxic dinoflagellate Alexandrium, which causes algal blooms and poisoning of shellfish
Creator	Shi, Lei Wang, Li Li, Zhiyong Li, Lin Alam, M Geng, Yuhuan Yamasaki, Shinji
Source	PMC
abstract	The marine dinoflagellate genus Alexandrium includes a number of species that produce potent neurotoxins responsible for paralytic shellfish poisoning, which in humans may cause muscular paralysis, neurological symptoms and, in extreme cases, death. Because of the genetic diversity of different genera and species, molecular tools may help to detect the presence of target microorganisms in marine field samples. Here we employed a loop-mediated isothermal amplification (LAMP) method for the rapid and simple detection of toxic Alexandrium species. A set of four primers were designed based upon the conserved region of the 5.8S rRNA gene of members of the genus Alexandrium. Using this detection system, toxic Alexandrium genes were amplified and visualized as a ladder-like pattern of bands on agarose gels under isothermal condition within 60 min. The LAMP amplicons were also directly visualized by eye in the reaction tube by the addition of SYBR Green I. This LAMP assay was 10-fold more sensitive than a conventional PCR method with a detection limit of 5 cells per tube when targeting DNA from Alexandrium minutum. The LAMP assay reported here indicates the potential usefulness of the technique as a valuable simple, rapid alternative procedure for the detection of target toxic Alexandrium species during coastal water monitoring.
has issue date	2008-05-01(xsd:dateTime)
bibo:doi	10.1111/j.1574-6968.2008.01074.x
bibo:pmid	18355290
has license	no-cc
sha1sum (hex)	7b63c70e8b5816e066a9b2fd958c3a0d2a9c1436
schema:url	https://doi.org/10.1111/j.1574-6968.2008.01074.x
resource representing a document's title	Loop-mediated isothermal amplification method for rapid detection of the toxic dinoflagellate Alexandrium, which causes algal blooms and poisoning of shellfish
has PubMed Central identifier	PMC7110188
has PubMed identifier	18355290
schema:publication	FEMS Microbiol Lett
resource representing a document's body	covid:7b63c70e8b5816e066a9b2fd958c3a0d2a9c1436#body_text
is schema:about of	named entity 'fold' named entity 'Alexandrium' named entity 'targeting' named entity 'Alexandrium' named entity 'cells' named entity 'sensitive' named entity 'species' named entity 'genera' named entity 'number' named entity 'molecular' named entity 'cation' named entity 'METHOD' named entity 'TOXIC' named entity 'POISONING' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'PRODUCE' named entity 'COASTAL WATER' named entity 'ADDITION' named entity 'GENUS' named entity 'SET' named entity 'LIKE' named entity 'WATER MONITORING' named entity 'MICROORGANISMS' named entity 'LAMP' named entity 'NEUROLOGICAL SYMPTOMS' named entity 'MOLECULAR' named entity 'MIN' named entity 'SYBR GREEN I' named entity 'VISUALIZED' named entity 'REACTION' named entity 'CONVENTIONAL' named entity 'NUMBER OF' named entity 'EXTREME' named entity 'DETECTION LIMIT' named entity 'DIFFERENT' named entity 'PRIMERS' named entity 'TARGET' named entity 'NEUROTOXINS' named entity 'TOXIC' named entity 'ALEXANDRIUM' named entity 'ALTERNATIVE' named entity 'RESPONSIBLE' named entity 'DETECTION' named entity 'SYSTEM' named entity 'TARGETING' named entity 'DNA' named entity 'METHOD' named entity 'CONSERVED REGION' named entity 'CELLS' named entity 'EMPLOYED' named entity 'CAUSES' named entity 'CASES' named entity 'ALEXANDRIUM MINUTUM' named entity 'HELP' named entity 'RAPID' named entity 'SENSITIVE' named entity 'SPECIES' named entity 'BANDS' named entity 'EYE' named entity 'SAMPLES' named entity 'TO DETECT' named entity 'INCLUDES' named entity 'MEMBERS' named entity 'ASSAY' named entity 'AMPLIFIED' named entity 'TECHNIQUE' named entity 'MUSCULAR PARALYSIS' named entity 'DINO' named entity 'ALGAL BLOOMS' named entity 'MEDIATED' named entity 'DETECTION' named entity 'RAPID' named entity 'PATTERN' named entity 'MARINE' named entity 'TUBE'

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