About: Chikungunya virus infectivity, RNA replication and non-structural polyprotein processing depend on the nsP2 protease’s active site cysteine residue

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About: Chikungunya virus infectivity, RNA replication and non-structural polyprotein processing depend on the nsP2 protease’s active site cysteine residue Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Chikungunya virus infectivity, RNA replication and non-structural polyprotein processing depend on the nsP2 protease’s active site cysteine residue
Creator	Merits, Andres Rausalu, Kai Utt, Age Ahola, Tero Quirin, Tania Das, Pratyush Varghese, Finny
Source	Medline; PMC
abstract	Chikungunya virus (CHIKV), genus Alphavirus, family Togaviridae, has a positive-stand RNA genome approximately 12 kb in length. In infected cells, the genome is translated into non-structural polyprotein P1234, an inactive precursor of the viral replicase, which is activated by cleavages carried out by the non-structural protease, nsP2. We have characterized CHIKV nsP2 using both cell-free and cell-based assays. First, we show that Cys478 residue in the active site of CHIKV nsP2 is indispensable for P1234 processing. Second, the substrate requirements of CHIKV nsP2 are quite similar to those of nsP2 of related Semliki Forest virus (SFV). Third, substitution of Ser482 residue, recently reported to contribute to the protease activity of nsP2, with Ala has almost no negative effect on the protease activity of CHIKV nsP2. Fourth, Cys478 to Ala as well as Trp479 to Ala mutations in nsP2 completely abolished RNA replication in CHIKV and SFV trans-replication systems. In contrast, trans-replicases with Ser482 to Ala mutation were similar to wild type counterparts. Fifth, Cys478 to Ala as well as Trp479 to Ala mutations in nsP2 abolished the rescue of infectious virus from CHIKV RNA transcripts while Ser482 to Ala mutation had no effect. Thus, CHIKV nsP2 is a cysteine protease.
has issue date	2016-11-15(xsd:dateTime)
bibo:doi	10.1038/srep37124
bibo:pmid	27845418
has license	cc-by
sha1sum (hex)	79b67a52cb7757c9fae9636913c30b5ee39e68af
schema:url	https://doi.org/10.1038/srep37124
resource representing a document's title	Chikungunya virus infectivity, RNA replication and non-structural polyprotein processing depend on the nsP2 protease’s active site cysteine residue
has PubMed Central identifier	PMC5109220
has PubMed identifier	27845418
schema:publication	Sci Rep
resource representing a document's body	covid:79b67a52cb7757c9fae9636913c30b5ee39e68af#body_text
is schema:about of	named entity 'VIRUS INFECTIVITY' named entity 'Ala' named entity 'protease' named entity 'CHIKV' named entity 'Ala' named entity 'CHIKV' named entity 'genome' named entity 'Chikungunya virus' named entity 'protease' named entity 'DEPEND' named entity 'PRECURSOR' covid:arg/79b67a52cb7757c9fae9636913c30b5ee39e68af named entity 'PROCESSING' named entity 'BASED' named entity 'FREE' named entity '27S' named entity 'CONTRAST' named entity 'SIMILAR' named entity 'ALMOST' named entity 'USING' named entity 'REQUIREMENTS' named entity 'STAND' named entity 'ACTIVATED' named entity 'STRUCTURAL POLYPROTEIN' named entity 'PROCESSING' named entity 'HAVE' named entity 'GENUS' named entity 'CHARACTERIZED' named entity 'NSP2' named entity 'RNA REPLICATION' named entity 'MUTATION' named entity 'RELATED' named entity 'SUBSTRATE' named entity 'STRUCTURAL' named entity 'CONTRIBUTE ' named entity 'CELL' named entity 'TOGAVIRIDAE' named entity 'OPEN' named entity 'STRUCTURAL POLYPROTEIN' named entity 'CHIKUNGUNYA VIRUS' named entity 'FOURTH' named entity 'ALA' named entity 'REPORTED' named entity 'RNA REPLICATION' named entity 'REPLICASE' named entity 'NSP2' named entity 'ALPHAVIRUS' named entity 'REPLICATION' named entity 'TRANS' named entity 'ACTIVE SITE' named entity 'PROTEASE ACTIVITY' named entity 'VIRAL' named entity 'NO EFFECT' named entity 'CHIKUNGUNYA VIRUS' named entity 'SITE OF' named entity 'RESCUE' named entity 'SYSTEMS' named entity 'RESIDUE' named entity 'NEGATIVE' named entity 'SECOND' named entity 'INACTIVE' named entity 'FIFTH' named entity 'MUTATIONS' named entity 'INFECTIOUS' named entity 'COMPLETELY' named entity 'PROTEASE' named entity 'IS A' named entity 'ABOLISHED' named entity 'POSITIVE' named entity 'INFECTED' named entity 'CARRIED' named entity 'WILD TYPE' named entity 'VIRUS' named entity 'PROTEASE' named entity 'RECENTLY'

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