About: Rapid Detection of Avian Infectious Bronchitis Virus by Reverse Transcriptase-Loop Mediated Isothermal Amplification

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About: Rapid Detection of Avian Infectious Bronchitis Virus by Reverse Transcriptase-Loop Mediated Isothermal Amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid Detection of Avian Infectious Bronchitis Virus by Reverse Transcriptase-Loop Mediated Isothermal Amplification
Creator	Chandrasekar, A Dhinakar, • Kumanan, • Raja, • Thangavelu, Raj
source	Medline; PMC
abstract	A reverse-transcription loop mediated isothermal amplification (RT-LAMP) was developed for rapid diagnosis of infectious bronchitis (IB) in poultry by targeting the spike protein 2 gene (S2). RT-LAMP primers were designed for IBV-S2 targets and optimized to run at 60 °C for 45 min. As compared with RT-PCR, RT-LAMP was 100 times more sensitive for IBV-S2 gene. RT-LAMP showed specific amplification with IB viral genome but not with other avian respiratory pathogens due to their mismatching with IBV-S2-RT-LAMP primers. RT-LAMP reaction products were visually detected by the addition of propidium iodide stain. Out of 102 field samples tested for detection of IBV, RT-LAMP detected IBV in 12 samples for S2 gene whereas RT-PCR detected IBV in six samples for S2 gene. The sensitivity of the RT-LAMP was 100 % and the specificity was 94 % for S2 gene. Since the developed RT-LAMP to detect IBV is simple, rapid, sensitive and specific, it can be a useful diagnostic tool for detection of IB in poultry in less equipped laboratories and in field conditions.
has issue date	2015-03-04(xsd:dateTime)
bibo:doi	10.1007/s40011-015-0490-4
bibo:pmid	32226206
has license	no-cc
sha1sum (hex)	798ed22f957a828b3f2b6f9e79e1e23214f4f54e
schema:url	https://doi.org/10.1007/s40011-015-0490-4
resource representing a document's title	Rapid Detection of Avian Infectious Bronchitis Virus by Reverse Transcriptase-Loop Mediated Isothermal Amplification
has PubMed Central identifier	PMC7100760
has PubMed identifier	32226206
schema:publication	Proc Natl Acad Sci India Sect B Biol Sci
resource representing a document's body	covid:798ed22f957a828b3f2b6f9e79e1e23214f4f54e#body_text
is schema:about of	named entity 'laboratories' named entity 'RT-PCR' named entity 'Since' named entity 'infectious bronchitis' named entity 'Detection' covid:arg/798ed22f957a828b3f2b6f9e79e1e23214f4f54e named entity 'detected' named entity 'avian' named entity 'rapid' named entity 'specific' named entity 'diagnosis' named entity 'sensitive' named entity 'diagnostic tool' named entity 'poultry' named entity 'poultry' named entity 'RT-PCR' named entity 'IBV' named entity 'primers' named entity 'isothermal' named entity 'Avian Infectious Bronchitis Virus' named entity 'IBV' named entity 'RT-PCR' named entity 'viral genome' named entity 'propidium iodide' named entity 'IBV' named entity 'India' named entity 'thermal cycler' named entity 'structural proteins' named entity 'Biotechnology' named entity 'fluorescence' named entity 'positive reaction' named entity 'Coimbatore' named entity 'fluorescence' named entity 'detection limit' named entity 'RNA' named entity 'nucleoprotein' named entity 'primers' named entity 'diagnostic test' named entity 'primers' named entity 'gene' named entity 'avian infectious bronchitis virus' named entity 'avian leukosis virus' named entity 'PCR-based' named entity 'chicken eggs' named entity 'National Centre' named entity 'denaturation' named entity 'gene' named entity 'viruses' named entity 'nucleoprotein' named entity 'Invitrogen' named entity 'propidium iodide' named entity 'GenBank' named entity 'viral disease' named entity 'epizooties' named entity 'primers' named entity 'highly conserved' named entity 'immunoperoxidase' named entity 'RNA' named entity 'avian leukosis virus' named entity 'gene' named entity 'IBV' named entity 'tissue samples' named entity 'nucleoprotein' named entity 'RNA' named entity 'RT-PCR' named entity 'Pune' named entity 'enzyme' named entity 'infectious bronchitis' named entity 'RFLP' named entity 'viral genome' named entity 'GenBank' named entity 'DNA strands' named entity 'primers' named entity 'IBV' named entity 'genome' named entity 'gene' named entity 'embryo'

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