About: Characterization of an internal ribosome entry site within mRNA 5 of murine hepatitis virus

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About: Characterization of an internal ribosome entry site within mRNA 5 of murine hepatitis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of an internal ribosome entry site within mRNA 5 of murine hepatitis virus
Creator	Siddell, S Thiel, V Jendrach, M
Source	Medline; PMC
abstract	The unique region of mRNA 5 of murine hepatitis virus contains two open reading frames, ORF 5a and ORF 5b. The downstream ORF 5b encodes the envelope (E) protein, an integral membrane protein of the virus. We have shown previously that the expression of ORF 5b is mediated by the internal entry of ribosomes. In the experiments reported here, we have used the in vitro translation of synthetic mRNAs to identify the region of mRNA 5 that mediates internal ribosome entry. Our results show that the 5′ border of the MHV mRNA 5 IRES element is located between nucleotides 227 and 244 in ORF 5a, while the 3′ border is located between nucleotides 140 and 172 in ORF 5b. The MHV mRNA 5 IRES element, therefore, contains not more than 280 nucleotides and encompasses the ORF 5b initiation codon. As evidenced by electrophoretic mobility shift assays, the IRES element of mRNA 5 interacts specifically with protein factors present in an L-cell lysate.
has issue date	2014-05-18(xsd:dateTime)
bibo:doi	10.1007/s007050050556
bibo:pmid	10416375
has license	no-cc
sha1sum (hex)	74b8617fa94a50282877737b821e151dc973ad24
schema:url	https://doi.org/10.1007/s007050050556
resource representing a document's title	Characterization of an internal ribosome entry site within mRNA 5 of murine hepatitis virus
has PubMed Central identifier	PMC7087331
has PubMed identifier	10416375
schema:publication	Arch Virol
resource representing a document's body	covid:74b8617fa94a50282877737b821e151dc973ad24#body_text
is schema:about of	named entity 'synthetic' named entity 'mRNA' named entity 'internal ribosome entry site' named entity 'INTERNAL RIBOSOME ENTRY SITE' named entity 'MRNA' named entity 'RIBOSOMES' named entity 'INITIATION CODON' named entity 'TO IDENTIFY' named entity 'PREVIOUSLY' named entity 'HERE' named entity 'BORDER' named entity 'PROTEIN' named entity 'ORF' named entity 'ENVELOPE' named entity '140' named entity 'CHARACTERIZATION' named entity 'IN VITRO TRANSLATION' named entity 'L-CELL' named entity 'UNIQUE' named entity 'CONTAINS' named entity 'RIBOSOME' named entity 'PROTEIN ' named entity 'ELECTROPHORETIC MOBILITY SHIFT ASSAYS' named entity 'INTEGRAL MEMBRANE PROTEIN' named entity '172' named entity 'REGION' named entity 'NUCLEOTIDES' named entity 'USED' named entity 'MEDIATED' named entity 'RESULTS' named entity 'ENTRY' named entity 'DOWNSTREAM' named entity 'HAVE' named entity 'CELL LYSATE' named entity 'VIRUS' named entity '244' named entity 'OUR' named entity 'NOT MORE THAN' named entity 'ELEMENT' named entity 'PRESENT' named entity 'IRES' named entity 'INTERNAL' named entity 'REPORTED' named entity 'EXPRESSION' named entity 'MRNA' named entity 'MURINE HEPATITIS VIRUS' named entity 'SYNTHETIC' named entity 'OPEN READING FRAMES' named entity 'EXPERIMENTS' named entity 'MURINE HEPATITIS VIRUS' named entity 'region' named entity 'protein' named entity 'initiation codon' named entity 'internal' named entity 'mRNAs' named entity 'located' named entity 'murine' named entity 'protein' named entity 'IRES' named entity 'ORF' named entity 'ORF' named entity 'The' named entity 'mRNA' named entity 'murine' named entity 'virus' named entity 'ORF' named entity 'mRNA' named entity 'protein' named entity 'integral membrane protein' named entity 'ribosomes' named entity 'ribosome' named entity 'murine' named entity 'hepatitis virus' named entity 'located' named entity 'mRNA' named entity 'coronavirus' named entity 'ORF' named entity 'primer' named entity 'ORF'

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