About: Quantitative analysis of an anti-viral immune escape compound ML-7 in feline plasma using ultra performance liquid chromatography/electrospray ionization mass spectrometry

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About: Quantitative analysis of an anti-viral immune escape compound ML-7 in feline plasma using ultra performance liquid chromatography/electrospray ionization mass spectrometry Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Quantitative analysis of an anti-viral immune escape compound ML-7 in feline plasma using ultra performance liquid chromatography/electrospray ionization mass spectrometry
Creator	Nauwynck, Hans Cherlet, Marc Croubels, Siska Daminet, Sylvie De Backer, Patrick Dewerchin, Hannah Gleich, Sabine
source	Elsevier; Medline; PMC
abstract	An analytical method for the quantitative measurement of ML-7, a product with possible anti-immune escape activity for feline infectious peritonitis virus (FIPV), in feline plasma was developed and validated. The sample preparation consists of a solid-phase extraction step on an MCX cartridge. ML-7 and ML-9, used as the internal standard for the analysis, were separated on an ACQUITY UPLC™ BEH C(18) reversed-phase column (1.7 μm, 50 mm × 2.1 mm I.D.), using isocratic elution with acetonitrile and 0.1% formic acid in water as the mobile phase. Both compounds were subsequently quantified in MRM mode on a Micromass(®) Quattro Premier™ XE triple quadrupole mass spectrometer. The use of a Thermo Scientific(®) Exactive™ orbitrap mass spectrometer made it possible to confirm the proposed fragmentation pattern of both ML-7 and ML-9. A validation study according to EC requirements was carried out, in which the method showed good performance. Linear behaviour was observed in the 1–2500 ng ml(−1) range, which is relevant for real sample analysis. Accuracy and precision were within the criteria requested by the EC requirements throughout this concentration range. Extraction recovery of ML-7 was 72%. Matrix effect for ML-7 was not higher than 8%. The method was successfully used for the monitoring of ML-7 in feline plasma after intravenous, subcutaneous or oral administration of an ML-7 formulation, for the determination of pharmacokinetic parameters, with a limit of quantification of 1 ng ml(−1) and a limit of detection of 0.4 ng ml(−1). The proposed method also shows good characteristics for the analysis of ML-7 in plasma of other animal species and human plasma.
has issue date	2012-09-15(xsd:dateTime)
bibo:doi	10.1016/j.jchromb.2012.08.014
bibo:pmid	22925719
has license	no-cc
sha1sum (hex)	741ad67bb03111d1e1c85c77b0c7f07b8137dd7a
schema:url	https://doi.org/10.1016/j.jchromb.2012.08.014
resource representing a document's title	Quantitative analysis of an anti-viral immune escape compound ML-7 in feline plasma using ultra performance liquid chromatography/electrospray ionization mass spectrometry
has PubMed Central identifier	PMC7105240
has PubMed identifier	22925719
schema:publication	J Chromatogr B Analyt Technol Biomed Life Sci
resource representing a document's body	covid:741ad67bb03111d1e1c85c77b0c7f07b8137dd7a#body_text
is schema:about of	named entity 'step' named entity 'pharmacokinetic' named entity 'virus' named entity 'study' named entity 'Matrix' named entity 'MCX' named entity 'cartridge' named entity 'VIRAL' named entity 'RECOVERY' named entity '1 MM' named entity 'REQUIREMENTS' named entity '0.1' named entity 'ANALYSIS' named entity 'PERFORMANCE' named entity 'ESCAPE' named entity 'EXTRACTION' named entity 'ISOCRATIC ELUTION' named entity 'LINEAR' named entity 'PRODUCT' named entity 'WATER' named entity 'FELINE INFECTIOUS PERITONITIS VIRUS' named entity '0.4' named entity 'REQUESTED' named entity 'SEPARATED' named entity 'COLUMN' named entity 'VALIDATION STUDY' named entity 'RANGE' named entity 'ML-7' named entity 'EXACTIVE' named entity 'FELINE' named entity 'ANIMAL SPECIES' named entity 'PROPOSED' named entity 'MODE' named entity 'MONITORING' named entity 'MATRIX' named entity 'REAL' named entity 'MASS SPECTROMETER' named entity 'CONFIRM' named entity 'ACCORDING' named entity 'PREMIER' named entity 'QUANTITATIVE ANALYSIS' named entity 'ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY' named entity 'ML-7' named entity 'USE OF' named entity 'FRAGMENTATION' named entity 'ORBITRAP' named entity 'PLASMA' named entity 'PATTERN' named entity 'BEH' named entity 'DETERMINATION' named entity 'HIGHER' named entity 'CONCENTRATION' named entity 'PHARMACOKINETIC PARAMETERS' named entity 'HUMAN PLASMA' named entity 'SAMPLE ANALYSIS' named entity 'MICROMASS' named entity 'LIMIT OF DETECTION' named entity 'EFFECT' named entity 'QUANTITATIVE' named entity 'STEP' named entity 'IONIZATION' named entity 'ANALYTICAL METHOD' named entity 'CARRIED' named entity 'USING' named entity 'ESCAPE' named entity 'COMPOUND' named entity 'FELINE' named entity 'IMMUNE' named entity 'MASS SPECTROMETRY' named entity 'PLASMA' named entity 'CHARACTERISTICS' named entity 'PRECISION' named entity 'ACCURACY' named entity 'TRIPLE QUADRUPOLE MASS SPECTROMETER' named entity 'ACQUITY UPLC'

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