About: Synergistic Roles of Eukaryotic Translation Elongation Factors 1Bγ and 1A in Stimulation of Tombusvirus Minus-Strand Synthesis

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About: Synergistic Roles of Eukaryotic Translation Elongation Factors 1Bγ and 1A in Stimulation of Tombusvirus Minus-Strand Synthesis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Synergistic Roles of Eukaryotic Translation Elongation Factors 1Bγ and 1A in Stimulation of Tombusvirus Minus-Strand Synthesis
Creator	Izotova, L Kinzy, T Nagy, P Sasvari, Z
Source	PMC
abstract	Host factors are recruited into viral replicase complexes to aid replication of plus-strand RNA viruses. In this paper, we show that deletion of eukaryotic translation elongation factor 1Bgamma (eEF1Bγ) reduces Tomato bushy stunt virus (TBSV) replication in yeast host. Also, knock down of eEF1Bγ level in plant host decreases TBSV accumulation. eEF1Bγ binds to the viral RNA and is one of the resident host proteins in the tombusvirus replicase complex. Additional in vitro assays with whole cell extracts prepared from yeast strains lacking eEF1Bγ demonstrated its role in minus-strand synthesis by opening of the structured 3′ end of the viral RNA and reducing the possibility of re-utilization of (+)-strand templates for repeated (-)-strand synthesis within the replicase. We also show that eEF1Bγ plays a synergistic role with eukaryotic translation elongation factor 1A in tombusvirus replication, possibly via stimulation of the proper positioning of the viral RNA-dependent RNA polymerase over the promoter region in the viral RNA template.These roles for translation factors during TBSV replication are separate from their canonical roles in host and viral protein translation.
has issue date	2011-12-15(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1002438
bibo:pmid	22194687
has license	cc-by
sha1sum (hex)	740a8ab7bf183f64284384c5dfaae0ae77d56558
schema:url	https://doi.org/10.1371/journal.ppat.1002438
resource representing a document's title	Synergistic Roles of Eukaryotic Translation Elongation Factors 1Bγ and 1A in Stimulation of Tombusvirus Minus-Strand Synthesis
has PubMed Central identifier	PMC3240602
has PubMed identifier	22194687
schema:publication	PLoS Pathog
resource representing a document's body	covid:740a8ab7bf183f64284384c5dfaae0ae77d56558#body_text
is schema:about of	named entity 'proteins' named entity 'strand' named entity 'plant' named entity 'reduces' named entity 'strand' named entity 'Tombusvirus' named entity 'Synthesis' named entity 'SYNTHESIS' named entity 'AID' named entity 'SEPARATE' named entity 'RNA VIRUSES' named entity 'BINDS' named entity 'PAPER' named entity 'LEVEL' named entity 'STRUCTURED' named entity 'HOST' named entity 'DELETION' named entity 'MINUS' named entity 'END' named entity 'PROPER' named entity 'OPENING' named entity 'RESIDENT' named entity 'TEMPLATE' named entity 'COMPLEX' named entity 'ADDITIONAL' named entity 'PLANT' named entity 'ROLES' named entity 'STRAINS' named entity 'ONE OF' named entity 'TOMBUSVIRUS' named entity 'VIRAL RNA' named entity 'TOMBUSVIRUS' named entity 'EUKARYOTIC TRANSLATION ELONGATION' named entity 'VIRAL PROTEIN' named entity 'PROTEIN TRANSLATION' named entity 'PROMOTER' named entity 'THESE' named entity 'STIMULATION' named entity 'SYNTHESIS' named entity 'POSITIONING' named entity 'PROTEINS' named entity 'POSSIBILITY OF' named entity 'REDUCING' named entity 'VIRAL' named entity 'ITS' named entity 'SYNERGISTIC' named entity 'PREPARED' named entity 'ROLE' named entity 'TOMATO BUSHY STUNT VIRUS' named entity 'CELL EXTRACTS' named entity 'THEIR' named entity 'TRANSLATION ELONGATION FACTOR' named entity 'ACCUMULATION' named entity 'ROLES' named entity 'REPLICATION' named entity 'REPLICASE' named entity 'YEAST' named entity 'DECREASES' named entity 'DEMONSTRATED' named entity 'RNA-DEPENDENT RNA POLYMERASE' named entity 'STRAND' named entity 'KNOCK DOWN' named entity 'STIMULATION' named entity 'PLAYS' named entity 'COMPLEXES' named entity 'IN VITRO' named entity 'EUKARYOTIC TRANSLATION ELONGATION' named entity 'REPEATED' named entity 'POSSIBLY' named entity 'SYNERGISTIC' named entity 'STRAND' named entity 'ELONGATION FACTORS' named entity 'MINUS' named entity 'TRANSLATION' named entity 'LACKING' named entity 'TEMPLATES' named entity 'UTILIZATION' named entity 'REGION'

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