About: Development and validation of a real-time PCR assay for specific and sensitive detection of canid herpesvirus 1

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About: Development and validation of a real-time PCR assay for specific and sensitive detection of canid herpesvirus 1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and validation of a real-time PCR assay for specific and sensitive detection of canid herpesvirus 1
Creator	Buonavoglia, Canio Decaro, Nicola Desario, Costantina Martella, Vito Lorusso, Eleonora Camero, Michele Amorisco, Francesca Sciarretta, Rossana Lucente, Maria Bellacicco, Anna
topic	covid:735319c8c59ffd63e489aa04075d18d049d2f351#this
Source	Elsevier; Medline; PMC
abstract	A TaqMan-based real-time PCR assay targeting the glycoprotein B-encoding gene was developed for diagnosis of canid herpesvirus 1 (CHV-1) infection. The established assay was highly specific, since no cross-reactions were observed with other canine DNA viruses, including canine parvovirus type 2, canine minute virus, or canine adenovirus types 1 and 2. The detection limit was 10(1) and 1.20 × 10(1) DNA copies per 10 μl(−1) of template for standard DNA and a CHV-1-positive kidney sample, respectively: about 1-log higher than a gel-based PCR assay targeting the thymidine kinase gene. The assay was also reproducible, as shown by satisfactory low intra-assay and inter-assay coefficients of variation. CHV-1 isolates of different geographical origins were recognised by the TaqMan assay. Tissues and clinical samples collected from three pups which died of CHV-1 neonatal infection were also tested, displaying a wide distribution of CHV-l DNA in their organs. Unlike other CHV-1-specific diagnostic methods, this quantitative assay permits simultaneous detection and quantitation of CHV-1 DNA in a wide range of canine tissues and body fluids, thus providing a useful tool for confirmation of a clinical diagnosis, for the study of viral pathogenesis and for evaluation of the efficacy of vaccines and antiviral drugs.
has issue date	2010-07-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.07.021
bibo:pmid	20674611
has license	no-cc
sha1sum (hex)	735319c8c59ffd63e489aa04075d18d049d2f351
schema:url	https://doi.org/10.1016/j.jviromet.2010.07.021
resource representing a document's title	Development and validation of a real-time PCR assay for specific and sensitive detection of canid herpesvirus 1
has PubMed Central identifier	PMC7112867
has PubMed identifier	20674611
schema:publication	J Virol Methods
resource representing a document's body	covid:735319c8c59ffd63e489aa04075d18d049d2f351#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/735319c8c59ffd63e489aa04075d18d049d2f351
is schema:about of	named entity 'parvovirus' named entity 'kinase' named entity 'assay' named entity 'viruses' named entity 'quantitation' named entity 'targeting' named entity 'evaluation' named entity 'origins' named entity 'kidney' named entity 'REAL-TIME PCR ' named entity 'CANINE MINUTE VIRUS' named entity 'CANINE PARVOVIRUS' named entity 'TOOL' named entity 'INCLUDING' named entity 'DIAGNOSTIC' named entity 'TYPE 2' named entity 'SATISFACTORY' named entity 'SAMPLE' named entity 'CANINE' named entity 'METHODS' named entity 'DNA' named entity 'RANGE' named entity 'TAQMAN' named entity 'CROSS' named entity 'KIDNEY' named entity 'CHV' named entity 'FOR DIAGNOSIS' named entity 'POSITIVE' named entity 'CLINICAL' named entity 'QUANTITATION' named entity 'DISPLAYING' named entity 'STUDY' named entity 'ENCODING' named entity 'GLYCOPROTEIN B' named entity 'GEOGRAPHICAL' named entity 'DISTRIBUTION' named entity 'COLLECTED' named entity 'CLINICAL DIAGNOSIS' named entity 'GEL' named entity 'DNA VIRUSES' named entity 'COPIES' named entity 'INTRA' named entity 'CANINE ADENOVIRUS' named entity 'REACTIONS' named entity 'DETECTION' named entity 'DIFFERENT' named entity 'ABOUT' named entity 'HIGHLY' named entity 'TEMPLATE' named entity 'ISOLATES' named entity 'ANTIVIRAL DRUGS' named entity 'LOW' named entity 'INFECTION' named entity 'PCR ASSAY' named entity 'CONFIRMATION OF' named entity 'NEONATAL INFECTION' named entity 'TYPES' named entity 'DETECTION' named entity 'DEVELOPMENT' named entity 'SIMULTANEOUS' named entity 'TARGETING' named entity 'QUANTITATIVE' named entity 'THYMIDINE KINASE GENE' named entity 'VIRAL PATHOGENESIS' named entity 'BASED' named entity '921' named entity 'VACCINES' named entity 'GENE' named entity 'BODY FLUIDS' named entity 'STANDARD'

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